{"created":"2023-05-15T14:36:01.342734+00:00","id":46359,"links":{},"metadata":{"_buckets":{"deposit":"a694d3fc-5475-4505-abae-5b22572973e1"},"_deposit":{"created_by":1,"id":"46359","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46359"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046359","sets":["1"]},"author_link":["461805","461808","461807","461809","461812","461806","461810","461814","461803","461811","461804","461813"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1316","bibliographicPageStart":"1309","bibliographicVolumeNumber":"1810","bibliographic_titles":[{"bibliographic_title":"Biochimica et Biophysica Acta. General Subjects"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: Gd-DTPA-enhanced magnetic resonance imaging (MRI) is a conventional method for non-invasive investigation of blood-brain-barrier (BBB) permeability in animal models. It allows the visualization of serious injury to the BBB. We developed a novel approach for detecting very small disruptions in BBB permeability induced by dietary cholesterol by using carbamoyl-PROXYL (CMP) as an MRI contrast probe.\n\\nMETHODS: Mice were separated into two groups: normal diet (ND-mice) and high cholesterol diet (CD-mice). MRI-signal dynamics, plasma cholesterol, matrix metalloproteinase (MMP-9, MMP-2), and the white blood cell profile were analyzed. For the MRI analysis, two regions-of-interest (ROI) were selected: brain (ROI-1) and surrounding area (ROI-2).\n\\nRESULTS: In the ROI-2 of ND-mice, CMP- or Gd-enhanced MRI-signal followed typical kinetics with a half-life of signal decay (t(1/2)) ~8 or ~15min, respectively. In CD-mice, the MRI-signal increased continuously without decay. In the ROI-1 of ND- and CD-mice, MRI-signal enhancement was not detected by Gd-DTPA. In the ROI-1 of ND-mice, CMP-induced MRI-signal enhancement was negligible, while in CD-mice, it was significant (t(1/2)>15min). Hypercholesterolemia increased the plasma levels of MMP-9 and neutrophils.\n\\nCONCLUSIONS: Hypercholesterolemia increases vascular permeability, which is mediated by MMP-9 and neutrophils.\n\\nGENERAL SIGNIFICANCE: Even very small disruptions in brain vascular permeability could be detected by CMP-enhanced MRI but not by Gd-DTPA-enhanced MRI.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbagen.2011.06.011","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0304-4165","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tomizawa, Atsuyuki"}],"nameIdentifiers":[{"nameIdentifier":"461803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii, Itsuko"}],"nameIdentifiers":[{"nameIdentifier":"461804","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhelev, Zhivko"}],"nameIdentifiers":[{"nameIdentifier":"461805","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio"}],"nameIdentifiers":[{"nameIdentifier":"461806","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibata, Sayaka"}],"nameIdentifiers":[{"nameIdentifier":"461807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bakalova-Zheleva, Rumiana"}],"nameIdentifiers":[{"nameIdentifier":"461808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitada, Mitsukazu"}],"nameIdentifiers":[{"nameIdentifier":"461809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 伊都子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461810","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461811","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 伊知男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461812","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柴田 さやか","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461813","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"バカロバ ルミアナ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461814","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-13"},"publish_date":"2012-07-13","publish_status":"0","recid":"46359","relation_version_is_last":true,"title":["Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:52:46.584790+00:00"}