{"created":"2023-05-15T14:36:01.130366+00:00","id":46354,"links":{},"metadata":{"_buckets":{"deposit":"f34c9b89-2135-482e-8d70-d6d91f57d576"},"_deposit":{"created_by":1,"id":"46354","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46354"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046354","sets":["1"]},"author_link":["461752","461754","461753","461755","461751"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1229","bibliographicPageStart":"1219","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Journal of Gastroenterology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background  \nDes-gamma-carboxy prothrombin (DCP) is an established tumor marker for hepatocellular carcinoma (HCC), but the precise mechanism of its production remains unknown. We have recently demonstrated that cytoskeletal rearrangement during the phenotypic changes involved in epithelial mesenchymal transition (EMT) plays a crucial role in DCP production through the impairment of vitamin K uptake. However, DCP production in long-lasting severe hypoxic conditions with nutrient deprivation, such as transarterial embolization, remains unknown.\nMethods  \nWe examined the effects of long-lasting hypoxia with nutrient deprivation, as well as the constitutive expression of hypoxia-inducible factor (HIF)-1-alpha, on EMT status, DCP production, and protein synthesis in human hepatoma cell lines by enzyme-linked immunosorbent assay, immunofluorescent studies, and western blotting. Immunohistochemistry findings for DCP, anti-hepatocyte paraffin 1 (Hep Par 1), and vimentin were examined using human resected HCC samples.\nResults  \nBoth severe hypoxia with nutrient deprivation and HIF-1-alpha transfection led to the cessation of DCP production, by attenuating protein synthesis through the hypophosphorylation of mammalian target of rapamycin and its effector proteins, indicative of a further phenotypic shift involving impaired liver-specific protein synthesis. In immunohistochemistry, the distribution of DCP- and Hep Par 1-positive HCC cells was mostly similar and vimentin-positive HCC cells were frequently observed in the areas that were negative for Hep Par 1 and/or DCP.\nConclusions  \nHCC cells produce DCP when they undergo mild phenotypic changes. However, when HCC cells adopt mesenchymal properties they lose their capacity for protein synthesis, and the production of DCP is attenuated. Building upon our previous works, it appears that DCP could be a unique tumor marker that reflects the stepwise phenotypic changes of HCC.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0944-1174","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Hideto"}],"nameIdentifiers":[{"nameIdentifier":"461751","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murata, Kazumoto"}],"nameIdentifiers":[{"nameIdentifier":"461752","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gotoh, Takaya"}],"nameIdentifiers":[{"nameIdentifier":"461753","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"461754","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤 孝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461755","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phenotype-dependent production of des-gamma-carboxy prothrombin in hepatocellular carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phenotype-dependent production of des-gamma-carboxy prothrombin in hepatocellular carcinoma"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-06-29"},"publish_date":"2012-06-29","publish_status":"0","recid":"46354","relation_version_is_last":true,"title":["Phenotype-dependent production of des-gamma-carboxy prothrombin in hepatocellular carcinoma"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:52:50.051047+00:00"}