{"created":"2023-05-15T14:36:00.202642+00:00","id":46336,"links":{},"metadata":{"_buckets":{"deposit":"c6e2fdcb-ebd1-4ac4-8167-bf7605a44286"},"_deposit":{"created_by":1,"id":"46336","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46336"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046336","sets":["1"]},"author_link":["461631","461632","461630","461625","461635","461627","461634","461626","461633","461628","461629"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"1050","bibliographicPageStart":"1045","bibliographicVolumeNumber":"103","bibliographic_titles":[{"bibliographic_title":"Cancer Science"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Poly(ADP-ribose) polymerase (PARP)-1 promotes base excision repair and DNA strand break repair. Inhibitors of PARP enhance the cytotoxic effects of gamma- and X-irradiation. We investigated the impact of PARP inhibition on the responses to gamma-irradiation (low LET (liner energy transfer) radiation) and carbon-ion irradiation (high LET radiation) in the human pancreatic cancer cell line MIA PaCa-2. Cell survival was assessed by colony formation assay after combination treatment with the PARP inhibitor AZD2281 and single fraction gamma-irradiation and carbon-ion irradiation (LET 13 and 70 keV/um). The DNA damage response (DDR) was assessed by pulse field gel electrophoresis (PFGE), Western blotting and flow cytometry. Treatment with a PARP inhibitor enhanced the cytotoxic effect of gamma-, LET 13 and LET 70 carbon-ion irradiation. Moreover, the radiosensitization effect was greater for LET 70 than for LET 13 irradiation. Prolonged and increased levels of gamma-H2AX were observed both after gamma- and carbon-ion irradiation in the presence of the PARP inhibitor. Enhanced level of phosphorylated-p53 (Ser-15) was observed after gamma-irradiation but not after carbon-ion irradiation. PARP inhibitor treatment induced S phase arrest and enhanced subsequent G2/M arrest both after gamma- and carbon-ion irradiation. These results suggest that the induction of S phase arrest through an enhanced DDR and a local delay in DNA double strand break processing by PARP inhibition caused sensitization to gamma- and carbon-ion irradiation. Taken together, PARP inhibitors might be applicable to a wide therapeutic range of LET radiation through their effects on the DDR.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-9032","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirai, Takahisa"}],"nameIdentifiers":[{"nameIdentifier":"461625","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shirai, Hidenori"}],"nameIdentifiers":[{"nameIdentifier":"461626","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"461627","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"461628","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasai, Keisuke"}],"nameIdentifiers":[{"nameIdentifier":"461629","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masutani, Mitsuko"}],"nameIdentifiers":[{"nameIdentifier":"461630","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平井 崇久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461631","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"白井 秀徳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笹井 啓資","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461634","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"益谷 美都子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461635","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Radiosensitization Effect of PARP Inhibition in Cells Exposed to Low and High LET Radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiosensitization Effect of PARP Inhibition in Cells Exposed to Low and High LET Radiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-06-05"},"publish_date":"2012-06-05","publish_status":"0","recid":"46336","relation_version_is_last":true,"title":["Radiosensitization Effect of PARP Inhibition in Cells Exposed to Low and High LET Radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:53:03.524531+00:00"}