@article{oai:repo.qst.go.jp:00046295,
 author = {Li, Ping and Zhou, Libin and Dai, Zhongying and Jin, Xiaodong and Liu, Xingou and Matsumoto, Yoshitaka and Furusawa, Yoshiya and Li, Qiang and Ｌｉ Ｐｉｎｇ and Ｊｉｎ Ｘｉａｏｄｏｎｇ and 松本 孔貴 and 古澤 佳也 and 李 強},
 issue = {4},
 journal = {Journal of Radiation Research},
 month = {Jul},
 note = {To understand how human tumor cells respond to the combined treatment with nocodazole and high LET radiation, alterations in cell cycle, mitotic disturbances and cell death were investigated in the present study. Human cervix carcinoma HeLa cells were exposed to nocodazole for 18 h immediately followed by high LET iron ion irradiation and displayed a sequence of events leading to DNA damages, mitotic aber- rations, interphase restitution and endocycle as well as cell death. A prolonged mitotic arrest more than 10 h was observed following nocodazole exposure, no matter the irradiation was present or not. The occur- rence of mitotic slippage following the mitotic arrest was only drug-dependent and the irradiation did not accelerate it. The amount of polyploidy cells was increased following mitotic slippage. No detectable G2 or G1 arrest was observed in cells upon the combined treatment and the cells reentered the cell cycle still harboring unrepaired cellular damages. This premature entry caused an increase of multipolar mitotic spin- dles and amplification of centrosomes, which gave rise to lagging chromosomal material, failure of cytoki- nesis and polyploidization. These mitotic disturbances and their outcomes confirmed the incidence of mitotic catastrophe and delayed apoptotic features displayed by TUNEL method after the combined treat- ment. These results suggest that the addition of high-LET iron ion irradiation to nocodazole enhanced mitotic catastrophe and delayed apoptosis in HeLa cells. These might be important cell death mechanisms involved in tumor cells in response to the treatment of antimitotic drug combined with high LET radiation.},
 pages = {481--489},
 title = {High LET radiation enhances nocodazole Induced cell death in HeLa cells through mitotic catastrophe and apoptosis.},
 volume = {52},
 year = {2011}
}