{"created":"2023-05-15T14:35:56.839417+00:00","id":46258,"links":{},"metadata":{"_buckets":{"deposit":"1770e438-4f5e-4d84-9f4c-38611b154b77"},"_deposit":{"created_by":1,"id":"46258","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46258"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046258","sets":["1"]},"author_link":["460686","460688","460689","460687","460684","460676","460673","460675","460679","460682","460680","460677","460678","460683","460674","460681","460685"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"185","bibliographicPageStart":"177","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives\nRadiolabeled Cu-diacetyl-bis (N4-methylthiosemicarbazone) ('Cu-ATSM), including 60/62/64Cu-ATSM, is a potential imaging agent of hypoxic tumors for positron emission tomography (PET). We have reported that 'Cu-ATSM is trapped in tumor cells under intracellular overreduced states, e.g., hypoxia. Here we evaluated 'Cu-ATSM as an indicator of intracellular overreduced states in mitochondrial disorders using cell lines with mitochondrial dysfunction.\n\\nMethods\nMitochondrial DNA-less p0206 cells; the parental 143B human osteosarcoma cells; the cybrids carrying mutated mitochondria from a patient of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD); and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia, and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH.\n\\nResults\np0206 cells showed higher 64Cu-ATSM uptake than control 143B cells under normoxia, whereas 64Cu-ATSM uptake was not significantly increased under hypoxia in p0206 cells. Additionally, 64Cu-ATSM uptake showed correlate change to the NADH and NADPH levels, but not oxygenic conditions. 2SD cells showed increased 64Cu-ATSM uptake under normoxia as compared with the control 2SA, and 64Cu-ATSM uptake followed NADH and NADPH levels, but not oxygenic conditions.\n\\nConclusions\n64Cu-ATSM accumulated in cells with overreduced states due to mitochondrial dysfunction, even under normoxia. We recently reported that 62Cu-ATSM-PET can visualize stroke-like episodes maintaining oxygen supply in MELAS patients. Taken together, our data indicate that 'Cu-ATSM uptake reflects overreduced intracellular states, despite oxygenic conditions; thus, 'Cu-ATSM would be a promising marker of intracellular overreduced states for disorders with mitochondrial dysfunction, such as MELAS, Parkinson's disease and Alzheimer's disease.\n\\nAbbreviations: Cu-ATSM, Cu-diacetyl-bis (N4-methylthiosemicarbazone); MELAS, mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes; mtDNA, mitochondrial DNA; PET, positron emission tomography","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.nucmedbio.2011.08.008","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshii, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"460673","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoneda, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"460674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikawa, Masamichi"}],"nameIdentifiers":[{"nameIdentifier":"460675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"460676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyono, Yasushi"}],"nameIdentifiers":[{"nameIdentifier":"460677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"460678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"460679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oyama, Nobuyuki"}],"nameIdentifiers":[{"nameIdentifier":"460680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazawa, Hidehiko"}],"nameIdentifiers":[{"nameIdentifier":"460681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"460682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"460683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 幸恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"清野 泰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 裕","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤林 康久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460689","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Radiolabeled Cu-ATSM as a novel indicator of overreduced intracellular state due to mitochondrial dysfunction: studies with mitochondrial DNA-less p0  cells and cybrids carrying MELAS mitochondrial DNA mutation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiolabeled Cu-ATSM as a novel indicator of overreduced intracellular state due to mitochondrial dysfunction: studies with mitochondrial DNA-less p0  cells and cybrids carrying MELAS mitochondrial DNA mutation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-02-01"},"publish_date":"2012-02-01","publish_status":"0","recid":"46258","relation_version_is_last":true,"title":["Radiolabeled Cu-ATSM as a novel indicator of overreduced intracellular state due to mitochondrial dysfunction: studies with mitochondrial DNA-less p0  cells and cybrids carrying MELAS mitochondrial DNA mutation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:54:00.713592+00:00"}