{"created":"2023-05-15T14:35:55.454763+00:00","id":46226,"links":{},"metadata":{"_buckets":{"deposit":"5a4d52b1-8111-402b-8e58-45d46a7b4c3d"},"_deposit":{"created_by":1,"id":"46226","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46226"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046226","sets":["1"]},"author_link":["460314","460315","460313","460310","460311","460312"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageEnd":"2275","bibliographicPageStart":"2267","bibliographicVolumeNumber":"317","bibliographic_titles":[{"bibliographic_title":"Experimental Cell Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells. Ku80 works as a heterodimer with Ku70. However, it is not yet clear whether KARP-1 forms a heterodimer with Ku70 and works as a heterodimer. Although KARP-1 appears to work in NHEJ, its physiological role remains unclear. In this study, we established and characterized EGFP-KARP-1-expressing xrs-6 cell lines, EGFP-KARP-1/xrs-6. We found that nuclear localization signal (NLS) of KARP-1 is localized in the C-terminal region. Our data showed that KARP-1 localizes within the nucleus in NLS-dependent and NLS-independent manner and forms a heterodimer with Ku70, and stabilizes Ku70. On the other hand, EGFP-KARP-1 could not perfectly complement the radiosensitivity and DSB repair activity of Ku80-deficient xrs-6 cells. Furthermore, KARP-1 could not accumulate at DSBs faster than Ku80, although EGFP-KARP-1 accumulates at DSBs. Our data demonstrate that the function of KARP-1 could not perfectly replace that of Ku80 in DSB repair, although KARP-1 has some biochemical properties, which resemble those of Ku80, and works as a heterodimer with Ku70. On the other hand, the number of EGFP-KARP-1-expressing xrs-6 cells showing pan-nuclear gamma-H2AX staining significantly increases following X-irradiation, suggesting that KARP-1 may have a novel role in DSB response","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-4827","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koike, Manabu"}],"nameIdentifiers":[{"nameIdentifier":"460310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yutoku, Yasutomo"}],"nameIdentifiers":[{"nameIdentifier":"460311","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Aki"}],"nameIdentifiers":[{"nameIdentifier":"460312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 学","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460313","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"湯徳 靖友","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460314","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 亜紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460315","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-19"},"publish_date":"2011-12-19","publish_status":"0","recid":"46226","relation_version_is_last":true,"title":["KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:54:22.495026+00:00"}