{"created":"2023-05-15T14:35:53.227301+00:00","id":46175,"links":{},"metadata":{"_buckets":{"deposit":"c82be862-aa7e-4544-9bd5-5a892c3af236"},"_deposit":{"created_by":1,"id":"46175","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46175"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046175","sets":["1"]},"author_link":["459776","459779","459774","459786","459791","459787","459777","459773","459780","459790","459778","459792","459772","459784","459788","459785","459783","459789","459782","459781","459775"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"14","bibliographicPageStart":"09","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"実験動物技術"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"放射線医学総合研究所で維持されている15マウス系統について遺伝学的モニタリングを実施するにあたり、従来の生化学的・免疫学的標識遺伝子を用いた方法からmicrosatellite markers（MSMs）を用いた方法に変えても可能であることを既に報告した。しかしアガロースゲル電気泳動法での目視による遺伝子型の判定がPCRプロダクトサイズの小差により困難な場合があった。その問題を改善すべく、自動電気泳動装置MultiNAを用いて電気泳動を行い、数値にて示されたPCRプロダクトサイズよりマウス系統間の遺伝子型の判定を行った。その結果、容易に遺伝子型を判定することができた。またその判定結果を既報のアガロースゲル電気泳動法での目視による結果と比較したところ、18座位10座位で更に精緻に判定可能であった。この結果よりMultiNAはMSMsによるマウス系統の遺伝学的モニタリングに有効なツールであることが確かめられた。","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0387-978X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"海野, あゆみ"}],"nameIdentifiers":[{"nameIdentifier":"459772","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"飯名, 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