@article{oai:repo.qst.go.jp:00046120,
 author = {Motohashi, Tsutomu and Yamanaka, Katsumasa and Chiba, Kairi and Miyajima, Kentaro and Aoki, Hitomi and Hirobe, Tomohisa and Kunisada, Takahiro and 廣部 知久},
 issue = {7},
 journal = {Developmental Dynamics},
 month = {Jul},
 note = {Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their
generation; after NC cells emerge from the neural tube, they are specified into the lineage-restricted precursors.
We analyzed the differentiation of early-stage NC-like cells derived from Sox10-IRES-Venus ES
cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the
Sox101/Kit2 cells and the Sox101/Kit1 cells, which were restricted in vivo to the neuron (N)-glial cell (G)
lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency.
We generated mice from the Sox10-IRES-Venus ES cells and analyzed the differentiation of their
NC cells. Both the Sox101/Kit2 cells and Sox101/Kit1 cells isolated from these mice formed colonies
containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells
retain multipotency even after the initial lineage-restricted stages.},
 pages = {1681--1693},
 title = {Neural Crest Cells Retain Their Capability for Multipotential Differentiation Even After Lineage-Restricted Stages},
 volume = {240},
 year = {2011}
}