{"created":"2023-05-15T14:35:49.712076+00:00","id":46094,"links":{},"metadata":{"_buckets":{"deposit":"152ba0d9-bcd2-4511-852c-9cdc7e06ffc1"},"_deposit":{"created_by":1,"id":"46094","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46094"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046094","sets":["1"]},"author_link":["458893","458888","458887","458892","458896","458894","458890","458891","458889","458886","458895"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1786","bibliographicPageStart":"1778","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Molecular Therapy"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glioblastoma multiforme (GM), the most frequent primary malignant brain tumor, is highly invasive due to the expression of proteases, including urokinase-type plasminogen activator (uPA). Here, we show the potential of our new and powerful recombinant Sendai virus (rSeV) showing uPA-specific cell-to-cell fusion activity [rSeV/dMFct14 (uPA2), named \"BioKnife\"] for GM treatment, an effect that was synergistically enhanced by arming BioKnife with the interferon-beta (IFN-beta) gene. BioKnife killed human GM cell lines efficiently in a uPA-dependent fashion, and this killing was prevented by PA inhibitor-1. Rat gliosarcoma 9L cells expressing both uPA and its functional receptor uPAR (9L-L/R) exhibited high uPA activity on the cellular surface and were highly susceptible to BioKnife. Although parent 9L cells (9L-P) were resistant to BioKnife and to BioKnife expressing IFN-beta (BioKnife-IFNbeta), cell-cell fusion of 9L-L/R strongly facilitated the expression of IFN-beta, and in turn, IFN-beta significantly accelerated the fusion activity of BioKnife. A similar synergy was seen in a rat orthotopic brain GM model with 9L-L/R in vivo; therefore, these results suggest that BioKnife-IFNbeta may have significant potential to improve the survival of GM patients in a clinical setting.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/mt.2010.138","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1525-0016","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Yuzo"}],"nameIdentifiers":[{"nameIdentifier":"458886","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwadate, Yasuo"}],"nameIdentifiers":[{"nameIdentifier":"458887","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Shigeyoshi"}],"nameIdentifiers":[{"nameIdentifier":"458888","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio"}],"nameIdentifiers":[{"nameIdentifier":"458889","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saeki, Naokatsu"}],"nameIdentifiers":[{"nameIdentifier":"458890","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yonemitsu, Yoshikazu"}],"nameIdentifiers":[{"nameIdentifier":"458891","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"458892","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川 祐三","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458893","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩立 康男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458894","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 茂芳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458895","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 伊知男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458896","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Urokinase-targeted fusion by oncolytic Sendai virus eradicates orthotopic glioblastomas by pronounced synergy with interferon-beta gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Urokinase-targeted fusion by oncolytic Sendai virus eradicates orthotopic glioblastomas by pronounced synergy with interferon-beta gene"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-06-06"},"publish_date":"2011-06-06","publish_status":"0","recid":"46094","relation_version_is_last":true,"title":["Urokinase-targeted fusion by oncolytic Sendai virus eradicates orthotopic glioblastomas by pronounced synergy with interferon-beta gene"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:55:56.015199+00:00"}