@article{oai:repo.qst.go.jp:00046070,
 author = {Wang, Yanling and Bing, Wang and Zhang, Hong and Li, Ning and Tanaka, Kaoru and Zhou, Xin and Chen, Ruping and Zhang, Xin and 王 冰 and 田中 薫 and Ｚｈａｎｇ Ｘｉｎ},
 issue = {5},
 journal = {Science China Physics, Mechanics and Astronomy},
 month = {Apr},
 note = {BRCA1 has been proposed to be tightly linked to the resistance of tumor
cells to ionizing radiation. The pathway leading to this phenomenon is not
yet clear. In this work, we investigated the role of BRCA1 in the apoptosis
regulation in response to carbon ion irradiation. We utilized three
different cancer cell lines with various states for BRCA1 and p53 to
identify the relationship between endogenous BRCA1 and the apoptosis-related
genes, and determine whether p53 function would affect the role of BRCA1 in
apoptosis regulation. By Western blot analysis, we found that Bax
expressions were not significantly changed after irradiation in all of three
cell lines. However, Bcl-2 expression showed an up-regulation by endogenous
BRCA1 regardless of p53 status. Moreover, the changes in Bcl-2 protein were
due to the increase in the transcriptional levels of Bcl-2 mRNA, based on
real-time PCR assay. At the same time, BRCA1-deficient cells appeared a
greater apoptosis susceptibility to irradiation when compared with
BRCA1-proficient cells. The results suggest that BRCA1 might exert
p53-independent regulative activities for Bcl-2, which seems account for the
low apoptosis susceptibility in BRCA1-proficient carcinomas.},
 pages = {916--922},
 title = {BRCA1 involves in regulation of Bcl-2 expression and apoptosis susceptibility to ionizing radiation},
 volume = {54},
 year = {2011}
}