@article{oai:repo.qst.go.jp:00046056,
 author = {Zhelev, Zhivko and Bakalova-Zheleva, Rumiana and Shibata, Sayaka and Veselina, Gadjeva and Aoki, Ichio and Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ and バカロバ ルミアナ and 柴田 さやか and 青木 伊知男},
 issue = {2},
 journal = {Trakia Journal of Sciences. Series Biomedical Sciences},
 month = {},
 note = {In the present study we propose a new diagnostic methodology for non-invasive imaging of tissue red/ox activity in intact healthy and cancer-bearing mammalians, which allows a differentiation of cancer development from normal (healthy) condition. The method is based on red/ox cycle of cellpermeable nitroxide radicals and their MRI contrast properties, which makes them useful molecular sensors for tissue red/ox activity. The nitroxide radical (which is characterized by T1 contrast) participates in electron-transfer reactions with the intracellular reactive oxygen species and reducing equivalents with formation of non-contrast intermediate products, which is accompanied with MRI signal decay. The half-life of MRI signal decay (1/2) was used as a marker of tissue red/ox activity to the nitroxide probe.
The experiments were conducted on healthy and cancer-bearing mice. The mice were under anesthesia during the MRI measurements. All measurements were conducted on 7 Tesla MRI.
In healthy mice, the half-life of MRI signal decay in the selected regions of interest (ROI; brain and surrounding tissues) was considered as a reference steady-state value, which is indicative of tissue red/ox activity in norm. In cancer-bearing mice, the half-life of MRI signal decay in the same or similar ROI was markedly different from this reference value. The results demonstrated that the normal (healthy) tissues possessed a significantly higher reduction activity to the nitroxide probe in comparison with cancer tissue, which could be an appropriate diagnostic marker for carcinogenesis.},
 pages = {1--5},
 title = {Magnetic resonance imaging of brain neuroblastoma based on nitroxide redox cycle},
 volume = {8},
 year = {2010}
}