{"created":"2023-05-15T14:35:47.348730+00:00","id":46040,"links":{},"metadata":{"_buckets":{"deposit":"286a15c1-9069-4062-84d0-0f0aec3c3bba"},"_deposit":{"created_by":1,"id":"46040","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46040"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046040","sets":["1"]},"author_link":["458239","458242","458241","458245","458234","458244","458240","458235","458243","458231","458237","458246","458238","458233","458236","458232"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"599","bibliographicPageStart":"592","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Journal of Nuclear Medicine"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The C2A domain of synaptotagmin I can target apoptotic cells by binding to exposed anionic phospholipids. The goal of this study was to synthesize and develop 18F-labeled C2A-glutathione-S-transferase (GST) as a molecular imaging probe for the detection of apoptosis and to assess the response of paclitaxel chemotherapy in VX2 rabbit lung cancer. Methods: 18F-C2A-GST was prepared by labeling C2A-GST with N-succinimidyl 4-18F-fluorobenzoate (18F-SFB). 18F-C2A-GST was confirmed by high-performance liquid chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The binding of 18F-C2A-GST toward apoptosis was validated in vitro using camptothecin-induced Jurkat cells. Biodistribution of 18F-C2A-GST was determined in mice by a dissection method and small-animal PET. Single-dose paclitaxel was used to induce apoptosis in rabbits bearing VX2 tumors (n = 6), and 2 VX2 rabbits without treatment served as control. 18F-C2A-GST PET was performed before and at 72 h after therapy, and 18F-FDG PET/CT was also performed before treatment. To confirm the presence of apoptosis, tumor tissue was analyzed and activated caspase-3 was measured. Results: 18F-C2A-GST was obtained with more than 95% radiochemical purity and was stable for 4 h after formulation. 18F-C2A-GST bound apoptotic cells specifically. Biodistribution in mice showed that 18F-C2A-GST mainly excreted from the kidneys and rapidly cleared from blood and nonspecific organs. High focal uptake of 18F-C2A-GST in the tumor area was determined after therapy, whereas no significant uptake before therapy was found in the tumor with 18F-FDG&#8211;avid foci. The maximum standardized uptake value after therapy was 0.47  +/-0.28, significantly higher than that in the control (0.009 +/- 0.001; P < 0.001). The apoptotic index was 79.81%  +/-8.73% in the therapy group, significantly higher than that in the control (5.03% +/- 0.81%; P < 0.001). Activated caspase-3 after paclitaxel treatment increased to 69.55%  +/-16.27% and was significantly higher than that in the control (12.26%  +/-5.39%; P < 0.001). Conclusion: 18F-C2A-GST was easily synthesized by conjugation with 18F-SFB and manifested a favorable biodistribution. Our results demonstrated the feasibility of 18F-C2A-GST for the early detection of apoptosis after chemotherapy in a VX2 lung cancer model that could imitate the human lung cancer initiation, development, and progress.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0161-5505","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"WANG, Feng"}],"nameIdentifiers":[{"nameIdentifier":"458231","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fang, Wei"}],"nameIdentifiers":[{"nameIdentifier":"458232","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"458233","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kumata, Katsushi"}],"nameIdentifiers":[{"nameIdentifier":"458234","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatori, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"458235","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamasaki, Tomoteru"}],"nameIdentifiers":[{"nameIdentifier":"458236","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanamoto, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"458237","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Kazutoshi"}],"nameIdentifiers":[{"nameIdentifier":"458238","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 峰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｆａｎｇ Ｗｅｉ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458241","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"熊田 勝志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458242","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"羽鳥 晶子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山崎 友照","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柳本 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 和年","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"458246","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of Chemotherapy Response in VX2 Rabbit Lung Cancer with 18F-Labeled C2A Domain of Synaptotagmin I","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of Chemotherapy Response in VX2 Rabbit Lung Cancer with 18F-Labeled C2A Domain of Synaptotagmin I"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-05-20"},"publish_date":"2011-05-20","publish_status":"0","recid":"46040","relation_version_is_last":true,"title":["Evaluation of Chemotherapy Response in VX2 Rabbit Lung Cancer with 18F-Labeled C2A Domain of Synaptotagmin I"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:56:34.747048+00:00"}