@article{oai:repo.qst.go.jp:00046031,
 author = {Hasegawa, Sumitaka and Koshikawa, Michiko and Saito, Shigeyoshi and Morokoshi, Yukie and Furukawa, Takako and Aoki, Ichio and Saga, Tsuneo and 長谷川 純崇 and 越川 道子 and 齋藤 茂芳 and 諸越 幸恵 and 古川 高子 and 青木 伊知男 and 佐賀 恒夫},
 issue = {9},
 journal = {International Journal of Cancer},
 month = {Jul},
 note = {Malignant mesothelioma (MM) is a fatal malignancy with a rapidly increasing incidence in industrialized countries because of the widespread use of asbestos in the past centuries. Early diagnosis of MM is critical for a better prognosis, but this is often difficult because of the lack of disease-specific diagnostic imaging. Here we report that manganese-enhanced magnetic resonance imaging (MEMRI) represents a promising approach for a more selective mesothelioma imaging by monitoring a high-level expression of manganese-superoxide dismutase (Mn-SOD), which is observed in many MM. We found that most human MM cells over-expressed Mn-SOD protein compared to human mesothelial cells, and that NCI-H226 human MM cells highly expressed Mn-SOD and augmented Mn accumulation when loaded with manganese chloride (MnCl(2)). The cells showed marked T(1)-signal enhancement on in vitro MRI after incubation with MnCl(2) because of the T(1) shortening effect of Mn(2+). H226 subcutaneous tumor was preferentially enhanced compared to a lung adenocarcinoma cell tumor and another human MM cell tumor in MnCl(2)-enhanced T(1)-weighted MR image (T(1)WI), correlating with their respective Mn-SOD expression levels. Moreover, in a more clinically relevant setting, H226 xenografted pleural tumor was markedly enhanced and readily detected by MEMRI using manganese dipyridoxyl diphosphate (MnDPDP), a clinically used contrast agent, as well as MnCl(2). Therefore, we propose that MEMRI can be a potentially powerful method for non-invasive detection of MM, with high spatial resolution and marked signal enhancement, by targeting Mn-SOD.},
 pages = {2138--2146},
 title = {Molecular imaging of mesothelioma by detection of manganese-superoxide dismutase activity using manganese-enhanced magnetic resonance imaging},
 volume = {128},
 year = {2010}
}