{"created":"2023-05-15T14:35:46.197992+00:00","id":46014,"links":{},"metadata":{"_buckets":{"deposit":"38d82c32-fbd2-4ad0-bec6-64c29879faa3"},"_deposit":{"created_by":1,"id":"46014","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46014"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046014","sets":["1"]},"author_link":["457951","457947","457952","457953","457946","457948","457949","457950"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"19","bibliographicPageEnd":"6465","bibliographicPageStart":"6456","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Nucleic Acids Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In eukaryotic cells, the Rad6/Rad18-dependent monoubiquitination of the proliferating cell nuclear antigen (PCNA) plays an essential role in the switching between replicatin and translesion DNA synthesis (TLS).The DNA polymerase PolHbinds to PCNA via a consensus C-terminal PCNA-interacting protein ( PIP) motif. It also specifically interacts with monoubiquitinated PCNA thanks to a recently identified ubiquitin-binding domain (UBZ).To investigate whether the TLS activity of PolHis always coupled to PCNA monoubiquitination, we monitor the ability of cell-free extracts to perform DNA synthesis across different types of lesions. We observe that a cis-syncyclobutane thymine dimer (TT-CPD),but not a N-2-2acetylaminouorene-guanine (G-AAF) adduct, is efficiently bypassed in extracts from Rad18-deficient cells, thus demonstrating the existence of a PolH -dependent and Rad18-independent TLS pathway .In addition ,by complementing PolH -deficient cells with PIP and UBZ mutants, we show that each of these domains contributes to PolH activity. The finding that the bypass of a CPD lesion in vitro does not require Ub-PCNA but nevertheless depends on the UBZ domain of PolH, reveals that this domain may play a novel role in the TLS process that is not related to the monoubiquitination status of  PCNA.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0305-1048","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Schmutz, Valerie"}],"nameIdentifiers":[{"nameIdentifier":"457946","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Janel-Bintz, Regine"}],"nameIdentifiers":[{"nameIdentifier":"457947","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wagner, Jerome"}],"nameIdentifiers":[{"nameIdentifier":"457948","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Biard, Denis"}],"nameIdentifiers":[{"nameIdentifier":"457949","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shiomi, Naoko"}],"nameIdentifiers":[{"nameIdentifier":"457950","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"P., Fuchs Robert"}],"nameIdentifiers":[{"nameIdentifier":"457951","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"M., Cordonnier Agnes"}],"nameIdentifiers":[{"nameIdentifier":"457952","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 尚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"457953","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Role of the ubiquitin-binding domain of PolH in RaD 18-independent translesion DNA synthesis in human cell extracts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of the ubiquitin-binding domain of PolH in RaD 18-independent translesion DNA synthesis in human cell extracts"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-03-08"},"publish_date":"2011-03-08","publish_status":"0","recid":"46014","relation_version_is_last":true,"title":["Role of the ubiquitin-binding domain of PolH in RaD 18-independent translesion DNA synthesis in human cell extracts"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:56:54.307579+00:00"}