@article{oai:repo.qst.go.jp:00045989,
 author = {Okada, Maki and Nakao, Ryuji and Hosoi, Rie and Zhang, Ming-Rong and Fukumura, Toshimitsu and Suzuki, Kazutoshi and Inoue, Osamu and 岡田 真希 and 中尾 隆士 and 細井 理恵 and 張 明栄 and 福村 利光 and 鈴木 和年 and 井上 修},
 issue = {1},
 journal = {Journal of Cerebral Blood Flow and Metabolism},
 month = {Apr},
 note = {The catecholamine, dopamine (DA), is synthesized from 3,4-dihydroxy-L-phenylalanine (L-DOPA) by aromatic L-amino acid decarboxylase (AADC). Dopamine metabolism is regulated by monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT). To measure dopaminergic metabolism, we used microdialysis with radiometric detection to monitor L-[beta-11C]DOPA metabolites in the extracellular space of the rat striatum. We also evaluated the effects of AADC, MAO, and COMT inhibitors on metabolite profiles. The major early species measured after administration of L-[beta-11C]DOPA were [11C]3,4-dihydroxyphenylacetic acid ([11C]DOPAC) and [11C]homovanillic acid ([11C]HVA) in a 1:1 ratio, which shifted toward [11C]HVA with time. An AADC inhibitor increased the uptake of L-[beta-11C]DOPA and L-3-O-methyl-[11C]DOPA and delayed the accumulation of [11C]DOPAC and [11C]HVA. The MAO and COMT inhibitors increased the production of [11C]3-methoxytyramine and [11C]DOPAC, respectively. These results reflect the L-DOPA metabolic pathway, suggesting that this method may be useful for assessing dopaminergic metabolism.},
 pages = {124--131},
 title = {Microdialysis with radiometric monitoring of L-[beta-11C]DOPA to assess dopaminergic metabolism: Effect of inhibitors of L-amino acid decarboxylase, monoamine oxidase, and catechol-O-methyltransferase on rat striatal dialysate.},
 volume = {31},
 year = {2010}
}