@article{oai:repo.qst.go.jp:00045959,
 author = {Liu, CuiHua and Kawata, Tetsuya and Shigematu, Naoyuki and A, Cucinotta Francesca and George, Kerry and Saitou, Masayoshi and Uno, Takashi and Isobe, Kouichi and Ito, Hisao and 劉 翠華 and 茂松 直之 and クシノッタ フランク and ジョージ ケリー and 斉藤 正好 and 宇野 隆 and 伊東 久夫},
 issue = {5},
 journal = {Radiation Research},
 month = {Nov},
 note = {Potentially lethal damage (PLD) and its repair were studied
in confluent human fibroblasts by analyzing the kinetics of
chromosome break rejoining and misrejoining in irradiated cells
that were either held in noncycling G0 phase or allowed to enter
G1 phase of the cell cycle immediately after 6 Gy irradiation.
Virally mediated premature chromosome condensation (PCC)
methods were combined with fluorescence in situ hybridization
(FISH) to study chromosomal aberrations in interphase. Flow
cytometry revealed that the vast majority of cells had not yet
entered S phase 15 h after release from G0. By this time some
95% of initially produced prematurely condensed chromosome
breaks had rejoined, indicating that most repair processes
occurred during G1. The rejoining kinetics of prematurely
condensed chromosome breaks was similar for each culture
condition. However, under noncycling conditions misrepair
peaked at 0.55 exchanges per cell, while under cycling conditions
(G1) it peaked at 1.1 exchanges per cell. At 12 h postirradiation,
complex-type exchanges were sevenfold more abundant for
cycling cells (G1) than for noncycling cells (G0). Since most
repair in G0/G1 occurs via the non-homologous end-joining
(NHEJ) process, increased PLD repair may result from
improved cell cycle-specific rejoining fidelity of the NHEJ
pathway.},
 pages = {566--573},
 title = {A Comparison of Chromosome Repair Kinetics in G0 and G1 Reveals that Enhanced Repair Fidelity under Noncycling Conditions Accounts for Increased Potentially Lethal Damage Repair},
 volume = {174},
 year = {2010}
}