{"created":"2023-05-15T14:35:39.183606+00:00","id":45853,"links":{},"metadata":{"_buckets":{"deposit":"a57cb870-d27b-4453-aa48-e037c1ac6553"},"_deposit":{"created_by":1,"id":"45853","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45853"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045853","sets":["1"]},"author_link":["456042","456044","456046","456047","456040","456041","456045","456043","456048"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"263","bibliographicPageStart":"258","bibliographicVolumeNumber":"395","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: We recently reported that gastrointestinal (GI) cancer cells can be reprogrammed to a pluripotent state by the ectopic expression of defined embryonic stem (ES)-like transcriptional factors. The induced pluripotent cancer (iPC) cells from GI cancer were sensitized to chemotherapeutic agents and differentiation-inducing treatment during a short-term culture, although a phenotype induced by long-term culture needs to be studied. METHODS: A long-term cultured (Lc)-iPC cells were produced in GI cancer cell lines by virus-mediated introduction of four ES-like genes-c-MYC, SOX2, OCT3/4, and KLF4-followed by a culture more than three months after iPC cells induction. An acquired state was studied by expression of immature-related surface antigens, Tra-1-60, Tra-1-81, Tra-2-49, and Ssea-4; and epigenetic trimethyl modification at lysine 4 of histone H3. Sensitivity to chemotherapeutic agents and tumorigenicity were studied in Lc-iPC cells. RESULTS: Whereas the introduction of defined factors of iPC cells once induced an immature state and sensitized cells to therapeutic reagents, the endogenous expression of the ES-like genes except for activated endogenous c-MYC was down-regulated in a long-term culture, suggesting a high magnitude of the reprogramming induction by defined factors and the requirement of therapeutic maintenance in Lc-iPC cells from cholangiocellular carcinoma HuCC-T1 cells, which harbor TP53(R175H) and KRAS(G12D). The Lc-iPC cells showed resistance to 5-fluorouracil in culture, and high tumorigenic ability with activated endogenous c-MYC in immunodeficient mice. CONCLUSION: The Lc-iPC cells from HuCC-T1 might be prone to an undesirable therapeutic response because of an association with the activated endogenous c-MYC. To consider the possible therapeutic approach in GI cancer, it would be necessary to develop a predictive method for evaluating the improper reprogramming-associated aggressive phenotype of iPC cells.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagai, Ken-ichi"}],"nameIdentifiers":[{"nameIdentifier":"456040","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii, Hideshi"}],"nameIdentifiers":[{"nameIdentifier":"456041","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"456042","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagano, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"456043","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekimoto, Mitsugu"}],"nameIdentifiers":[{"nameIdentifier":"456044","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Doki, Yuichiro"}],"nameIdentifiers":[{"nameIdentifier":"456045","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Masaki"}],"nameIdentifiers":[{"nameIdentifier":"456046","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"456047","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 俊行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"456048","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-12"},"publish_date":"2010-07-12","publish_status":"0","recid":"45853","relation_version_is_last":true,"title":["Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:58:50.311552+00:00"}