@article{oai:repo.qst.go.jp:00045801,
 author = {Ishihara, Hiroshi and Tanaka, Izumi and Yakumaru, Haruko and Chikamori, Minoru and Ishihara, Fumiko and Tanaka, Mika and Ishiwata, Akiko and Kurematsu, Ayako and Sato, Akiko and Ueda, Junichi and Akashi, Makoto and 石原 弘 and 田中 泉 and 薬丸 晴子 and 近森 穣 and 石原 文子 and 田中 美香 and 石渡 明子 and 槫松 文子 and 佐藤 明子 and 上田 順市 and 明石 真言},
 issue = {3},
 journal = {Journal of Radiation Research},
 month = {May},
 note = {Molecular mechanisms of intracellular response after DNA-damage by exposure to ionizing radiation have been studied. In the case of cells isolated from living body of human and experimental animals, alteration of the responsiveness by physiological oscillation such as circadian rhythm must be considered. To examine the circadian variation in the response of p53-responsible genes p21, mdm2, bax, and puma, we established a method to quantitate their mRNA levels with high reproducibility and accuracy based on real-time RT-PCR and compared the levels of responsiveness in mouse hemocytes after diurnal irradiation to that after nocturnal irradiation. Augmentations of p21 and mdm2 mRNA levels with growth-arrest and of puma mRNA before apoptosis were confirmed by time-course experiment in RAW264.7, and dose-dependent increases in the peak levels of all the RNA were shown. Similarly, the relative RNA levels of p21, mdm2, bax, and puma per GAPDH also increased dose-dependently in peripheral blood and bone marrow cells isolated from whole-body-irradiated mice. Induction levels of all messages reduced by half after nighttime irradiation as compared with daytime irradiation in blood cells. In marrow cells, nighttime irradiation enhanced the p21 and mdm2 mRNA levels than daytime irradiation. No significant difference in bax or puma mRNA levels was observed between nighttime and daytime irradiation in marrow cells. This suggests that early-stage cellular responsiveness in DNA damage-induced genes is modulated between diurnal and nocturnal irradiation.},
 pages = {265--275},
 title = {Circadian Transitions in Radiation Dose-Dependent Augmentation of mRNA Levels for DNA Damage-induced Genes Elicited by Accurate real-time RT-PCR Quantification.},
 volume = {51},
 year = {2010}
}