{"created":"2023-05-15T14:35:36.500600+00:00","id":45791,"links":{},"metadata":{"_buckets":{"deposit":"4c1477a5-21fd-4d1d-b516-b96c72301cc7"},"_deposit":{"created_by":1,"id":"45791","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45791"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045791","sets":["1"]},"author_link":["455251","455249","455250","455246","455247","455248"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"650","bibliographicPageStart":"645","bibliographicVolumeNumber":"173","bibliographic_titles":[{"bibliographic_title":"Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, we attempted to clarify the influence of the DNA repair genes RAD54 and KU70, components of the homologous recombination (HR) and non-homologous end-joining (NHEJ) pathways, respectively, on apoptosis induced by 1 Gy (low-dose) and 5 Gy (high-dose) irradiation. All experiments were performed using chicken B-lymphocyte DT40 cells and the DNA repair-deficient cell lines KU70-/-, RAD54-/- and KU70-/-/ RAD54-/-. Morphological changes were detected by fluorescence methods, and the sub-G1 fraction and the activated caspases in DT40 cells were analyzed by flow cytometry. Irradiation with 1 Gy significantly increased the level of apoptosis in cells with the defective DNA repair genes, with the maximum apoptosis occurring in double mutant cells, KU70-/-/RAD54-/-, demonstrating that 1 Gy is enough to induce apoptosis in DNA repair-deficient DT40 cells, and that KU70 and RAD54 must have almost the same role in low-dose radiation-induced apoptosis. After 5 Gy, fast induction of apoptosis, within 2 h, was seen in both wild-type cells and RAD54-/- cells, indicating that functional KU70 must be important for the rescue of the cells from the induction of fast apoptosis.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1667/rr1919.1","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0033-7587","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Momoko"}],"nameIdentifiers":[{"nameIdentifier":"455246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakura, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"455247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"455248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 桃子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"455249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高倉 かほる","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"455250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"455251","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparison of the Kinetics of Radiation-Induced Apoptosis in DT40 Cells Irradiated with Low and High Doses of X Rays","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparison of the Kinetics of Radiation-Induced Apoptosis in DT40 Cells Irradiated with Low and High Doses of X Rays"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-05-13"},"publish_date":"2010-05-13","publish_status":"0","recid":"45791","relation_version_is_last":true,"title":["Comparison of the Kinetics of Radiation-Induced Apoptosis in DT40 Cells Irradiated with Low and High Doses of X Rays"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:59:33.343411+00:00"}