{"created":"2023-05-15T14:35:35.128974+00:00","id":45760,"links":{},"metadata":{"_buckets":{"deposit":"481331ac-5a4d-4de8-9e47-b17356fe1a69"},"_deposit":{"created_by":1,"id":"45760","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45760"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045760","sets":["1"]},"author_link":["454857","454867","454873","454870","454863","454866","454875","454865","454872","454869","454856","454859","454861","454874","454862","454868","454860","454871","454864","454858"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"493","bibliographicPageStart":"488","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Synapse"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the central nervous system, translocator protein\n(TSPO) (18 kDa) (Papadopoulos et al., 2006), formerly\nnamed peripheral-type benzodiazepine receptor\n(PBR), is located in the microglial cells of the brain\n(Braestrup et al., 1977; Stephenson et al., 1995).\nMany studies have elucidated the relationship\nbetween TSPO and neurodegenerative diseases, such\nas Alzheimer's disease (Maeda et al., 2007; Yasuno\net al., 2008), and stroke-induced brain injury (Rojas\net al., 2007). To image TSPO in vivo, a number of\nnew ligands have been labeled with the positron emitters\n11C and 18F (Chauveau et al., 2008). We have\ndeveloped [11C]DAA1106 (Zhang et al., 2003; Maeda\net al., 2004), [18F]FEDAA1106 (Zhang et al., 2004),\nand [11C]AC-5216 (Zhang et al., 2007) for clinical\nimaging of TSPO in the brain. We recently synthesized\nand evaluated N-benzyl-N-[11C]methyl-2-(7-\nmethyl-8-oxo-2-phenyl-7, 8-dihydro-9H-purin-9-yl)acetamide\nwith a specific activity of 37–110 GBq/lmol for\nthe imaging of TSPO (Yanamoto et al., 2009).\nThe aim of this study was to synthesize N-benzyl-\nN-methyl-2-(7-[11C]methyl-8-oxo-2-phenyl-7,8-dihydro-\n9H-purin-9-yl)acetamide ([11C]DAC) with ultra-high\nspecific activity (average 4560 GBq/lmol), and to\ncharacterize specific binding to TSPO in the rat brain\nafter ischemia. Using [11C]DAC of high specific activity,\nwe expected to acquire high uptake ratios\nbetween the ipsilateral and contralateral sides and\nhigher binding potential for TSPO from autoradiographic\nand PET images of infarct brains. Here, we\nlabeled the methyl group of purine ring of DAC to\nachieve ultra-high specific activity using [11C]CH3I\nthat was prepared by iodination of [11C]CH4 (Noguchi\nand Suzuki, 2003; Noguchi et al., 2008). We\nperformed comparative evaluation using [11C]DAC\nwith middle specific activity and low specific activity\nto elucidate the effect of specific activity on the in\nvitro and in vivo specific binding of [11C]DAC to\nTSPO in an infarct rat brain after ischemia. In this\narticle we use the terms ''high SA,'' ''middle SA,'' and\n''low SA'' to refer to specific activities of 3540–5580,\n29–52, and 0.3–0.4 GBq/lmol, respectively.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0887-4476","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yui, Joji"}],"nameIdentifiers":[{"nameIdentifier":"454856","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatori, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"454857","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanamoto, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"454858","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takei, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"454859","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nengaki, Nobuki"}],"nameIdentifiers":[{"nameIdentifier":"454860","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kumata, Katsushi"}],"nameIdentifiers":[{"nameIdentifier":"454861","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawamura, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"454862","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamasaki, Tomoteru"}],"nameIdentifiers":[{"nameIdentifier":"454863","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Kazutoshi"}],"nameIdentifiers":[{"nameIdentifier":"454864","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"454865","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"由井 譲二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454866","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"羽鳥 晶子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454867","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柳本 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武井 誠","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"念垣 信樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"熊田 勝志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河村 和紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山崎 友照","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 和年","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454875","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-03"},"publish_date":"2010-03-03","publish_status":"0","recid":"45760","relation_version_is_last":true,"title":["Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:59:55.553796+00:00"}