{"created":"2023-05-15T14:35:35.038512+00:00","id":45758,"links":{},"metadata":{"_buckets":{"deposit":"bd906dc0-0818-49d5-8e99-57a7e678c487"},"_deposit":{"created_by":1,"id":"45758","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45758"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045758","sets":["1"]},"author_link":["454834","454831","454841","454842","454832","454837","454835","454830","454836","454838","454829","454840","454839","454827","454833","454828"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"241","bibliographicPageStart":"229","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Oncology Biology Physics"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PURPOSE: We examined biological responses of human glioma cells to irradiation with carbon ion beams (C-ions). \nMETHODS AND MATERIALS: A human glioma-derived cell line, NP-2, was irradiated with C-ions. Apoptotic cell nuclei were stained with Hoechst 33342. Induction of autophagy was examined either by staining cells with monodansylcadaverine (MDC) or by Western blotting to detect conversion of microtuble-associated protein light chain 3 (MAP-LC3) (LC3-I) to the membrane-bound form (LC3-II). Cellular senescence markers including induction of senescence-associated beta-galactosidase (SA-beta-gal) were examined. The mean telomere length of irradiated cells was determined by Southern blot hybridization. Expression of tumor suppressor p53 and cyclin/cyclin-dependent kinase inhibitor p21(WAF1/CIP1) in the irradiated cells was analyzed by Western blotting. \nRESULTS: When NP-2 cells were irradiated with C-ions at 6 Gy, the major population of the cells died of apoptosis and autophagy. The residual fraction of attached cells (<1% of initially irradiated cells) could not form a colony: however, they showed a morphological phenotype consistent with cellular senescence, that is, enlarged and flattened appearance. The senescent nature of these attached cells was further indicated by staining for SA-beta-gal. The mean telomere length was not changed after irradiation with C-ions. Phosphorylation of p53 at serine 15 as well as the expression of p21(WAF1/CIP1) was induced in NP-2 cells after irradiation. Furthermore, we found that irradiation with C-ions induced cellular senescence in a human glioma cell line lacking functional p53. \nCONCLUSIONS: Irradiation with C-ions induced apoptosis, autophagy, and cellular senescence in human glioma cells.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijrobp.2009.08.054","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0360-3016","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jinno-Oue, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"454827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Nobuaki"}],"nameIdentifiers":[{"nameIdentifier":"454828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hamada, Nobuyuki"}],"nameIdentifiers":[{"nameIdentifier":"454829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Seiichi"}],"nameIdentifiers":[{"nameIdentifier":"454830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"454831","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shinagawa, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"454832","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtsuki, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"454833","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Takahisa"}],"nameIdentifiers":[{"nameIdentifier":"454834","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"N., Saha Manujendra"}],"nameIdentifiers":[{"nameIdentifier":"454835","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"S., Hoque Ariful"}],"nameIdentifiers":[{"nameIdentifier":"454836","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Islam, Salequl"}],"nameIdentifiers":[{"nameIdentifier":"454837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kogure, Kimitaka"}],"nameIdentifiers":[{"nameIdentifier":"454838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Funayama, Tomoo"}],"nameIdentifiers":[{"nameIdentifier":"454839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Yasuhiko"}],"nameIdentifiers":[{"nameIdentifier":"454840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hoshino, Hiroo"}],"nameIdentifiers":[{"nameIdentifier":"454841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"浜田 信行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454842","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Irradiation with carbon ion beams induces apoptosis, autophagy, and cellular senescence in a human glioma-derived cell line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Irradiation with carbon ion beams induces apoptosis, autophagy, and cellular senescence in a human glioma-derived cell line"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-26"},"publish_date":"2010-02-26","publish_status":"0","recid":"45758","relation_version_is_last":true,"title":["Irradiation with carbon ion beams induces apoptosis, autophagy, and cellular senescence in a human glioma-derived cell line"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:59:57.038162+00:00"}