{"created":"2023-05-15T14:35:33.032413+00:00","id":45712,"links":{},"metadata":{"_buckets":{"deposit":"25c8a3ed-8dc1-4fe1-8980-79a55e64dc83"},"_deposit":{"created_by":1,"id":"45712","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45712"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045712","sets":["1"]},"author_link":["454367","454364","454362","454369","454365","454357","454355","454363","454368","454366","454370","454372","454359","454361","454360","454358","454371","454356"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"265","bibliographicPageStart":"257","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"Cancer Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We recently reported a novel suppressive effect of sodium orthovanadate (vanadate) on the DNA-binding activity of p53. Here, we showed that vanadate had a more potent antiapoptotic activity than three other chemical p53 inhibitors, including pifithrin-alpha. Although the other agents inhibited p53's transcriptional activity, they did not suppress p53-dependent apoptosis in irradiated MOLT-4 cells. To investigate the cause for the different effects of vanadate and the other inhibitors, we chose PFT-alpha and PFT-mu (an inhibitor of the p53-mediated transcription-independent apoptotic pathway), as references, and determined their and vanadate's effect on p53-mediated apoptosis, with a focus on the transcription-independent pathway. We found that vanadate suppressed the p53-associated apoptotic events at the mitochondria, including the loss of mitochondrial membrane potential, the conformational change of Bax and Bak, the mitochondrial translocation of p53, and p53's interaction with Bcl-2. Vanadate also suppressed the apoptosis-inducing activity of a mitochondrially targeted temperature-sensitive p53 in stable transfectants of the SaOS-2 cell line. Finally, we tested vanadate's potential as a radioprotector. Vanadate completely protected mice from a sublethal dose of 8 Gy and partially from a lethal dose of 12 Gy. Our data demonstrate that vanadate can suppress both the transcription-dependent and the transcription-independent p53 pathways, and suggest that both pathways must be inhibited to completely block p53-mediated apoptosis.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1158/0008-5472.can-08-3771","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0008-5472","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Morita, Akinori"}],"nameIdentifiers":[{"nameIdentifier":"454355","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Shinichi"}],"nameIdentifiers":[{"nameIdentifier":"454356","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bing, Wang"}],"nameIdentifiers":[{"nameIdentifier":"454357","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"454358","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Norio"}],"nameIdentifiers":[{"nameIdentifier":"454359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Shin"}],"nameIdentifiers":[{"nameIdentifier":"454360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Azusa"}],"nameIdentifiers":[{"nameIdentifier":"454361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nanao, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"454362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohya, Souichirou"}],"nameIdentifiers":[{"nameIdentifier":"454363","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhu, Jin"}],"nameIdentifiers":[{"nameIdentifier":"454364","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshino, Minako"}],"nameIdentifiers":[{"nameIdentifier":"454365","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Enomoto, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"454366","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"454367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Funatsu, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"454368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hosoi, Yoshio"}],"nameIdentifiers":[{"nameIdentifier":"454369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikekita, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"454370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中 薫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454372","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sodium orthovanadate inhibits p53-mediated apoptosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sodium orthovanadate inhibits p53-mediated apoptosis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-01-05"},"publish_date":"2010-01-05","publish_status":"0","recid":"45712","relation_version_is_last":true,"title":["Sodium orthovanadate inhibits p53-mediated apoptosis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:00:32.634705+00:00"}