{"created":"2023-05-15T14:35:31.208123+00:00","id":45679,"links":{},"metadata":{"_buckets":{"deposit":"5d185b69-fd2e-4fbe-988f-dfb3607a263a"},"_deposit":{"created_by":1,"id":"45679","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45679"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045679","sets":["1"]},"author_link":["453944","453945","453942","453950","453947","453941","453948","453949","453943","453940","453946"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"494","bibliographicPageStart":"487","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For sequential observation of radiation-induced apoptosis in a living embryo, we injected Acridine Orange (AO) solution into the yolk of embryo and visualized radiation-induced apoptosis in developing optic tectum (OT). Medaka embryos at stage 28, when neural cells proliferate rapidly in the OT, were irradiated with 5 Gy X-rays which is non-lethal dose for irradiated embryos at hatching. The irradiated embryos hatched normally without morphological abnormalities in their brains, even though a large number of apoptotic cells were induced transiently in OT. By yolk injection, apoptotic cells in OT were distinguished as AO-positive small nuclei at 3 h after irradiation. At 8-10 h after irradiation, AO-positive rosette-shaped clusters were obviously distinguished in marginal tectal regions of OT where cells are proliferating intensely. The AO-positive clusters became bigger and more obvious, but its number did not increase up to 24 h after irradiation and completely disappeared up to 49 h after irradiation. This characteristic appearance of the AO-positive nuclei/clusters is in a good agreement with our previous results based on the examination of fixed specimens stained with AO by injection into the peri-vitelline space, suggesting that the AO-yolk injection method is highly reliable for detecting apoptotic cells in living embryos. The live imaging of apoptotic cells in developing Medaka embryos by AO-yolk injection method is expected to reveal more in details of dynamics of the apoptotic responses in the irradiated brain and other tissues.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasuda, Takako"}],"nameIdentifiers":[{"nameIdentifier":"453940","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Shoji"}],"nameIdentifiers":[{"nameIdentifier":"453941","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuuji"}],"nameIdentifiers":[{"nameIdentifier":"453942","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asaka, Tomomi"}],"nameIdentifiers":[{"nameIdentifier":"453943","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hidaka, Masayuki"}],"nameIdentifiers":[{"nameIdentifier":"453944","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"453945","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anzai, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"453946","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitani, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"453947","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 裕二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453948","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"保田 浩志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453949","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安西 和紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453950","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Live Imaging of Radiation-Induced Apoptosis by Yolk Injection of Acridine Orange in the Developing Optic Tectum of Medaka","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Live Imaging of Radiation-Induced Apoptosis by Yolk Injection of Acridine Orange in the Developing Optic Tectum of Medaka"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-07"},"publish_date":"2009-12-07","publish_status":"0","recid":"45679","relation_version_is_last":true,"title":["Live Imaging of Radiation-Induced Apoptosis by Yolk Injection of Acridine Orange in the Developing Optic Tectum of Medaka"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:00:56.113553+00:00"}