{"created":"2023-05-15T14:35:30.943696+00:00","id":45675,"links":{},"metadata":{"_buckets":{"deposit":"9c5bb046-e455-4fa6-9549-94ccd393ae6c"},"_deposit":{"created_by":1,"id":"45675","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45675"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045675","sets":["1"]},"author_link":["453914","453913","453916","453915","453917"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"658","bibliographicPageStart":"654","bibliographicVolumeNumber":"390","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have previously studied genomic copy number changes and global gene expression patterns in rat osteosarcomas (OS) induced by the bone-seeking alpha emitter (238)Pu by comparative genomic hybridization (CGH) and oligonucleotide microarray analyses, respectively. Among the previously identified genes that were down-regulated in radiation-induced rat OS tumors, Cited2 (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2) and Akap12 (a kinase anchoring protein, also known as src-suppressed C-kinase substrate, SSeCKS) genes mapped to the most frequently lost regions on chromosome 1p. In the present study, relative copy number losses of Cited2 and Akap12 genes were observed in 8 of 15 (53%) and 10 of 15 (67%) tumors by quantitative PCR analysis. Loss of Cited2 and Akap12 in the tumors was confirmed at the levels of mRNA and protein expression by quantitative RT-PCR and immunoblot analyses, respectively. These results indicate that Cited2 and Akap12 are silenced in radiation-induced OS, and therefore are novel candidate tumor-suppressor genes of this tumor.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2009.10.022","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Daino, Kazuhiro"}],"nameIdentifiers":[{"nameIdentifier":"453913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chevillard, Sylvie"}],"nameIdentifiers":[{"nameIdentifier":"453914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"453915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"臺野 和広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"シュビヤール シルビー","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453917","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-29"},"publish_date":"2009-11-29","publish_status":"0","recid":"45675","relation_version_is_last":true,"title":["Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:00:58.831492+00:00"}