{"created":"2023-05-15T14:35:28.970114+00:00","id":45645,"links":{},"metadata":{"_buckets":{"deposit":"41636d65-a12d-4dd7-bf38-ea65e7898b39"},"_deposit":{"created_by":1,"id":"45645","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45645"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045645","sets":["1"]},"author_link":["453593","453589","453583","453592","453587","453594","453591","453590","453586","453584","453585","453582","453588"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"486","bibliographicPageStart":"477","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heavy ion beams are a high-LET radiation that has greater biological effect than electron beams or X-rays. However, little is known about the effect of heavy ion beams on the proliferation and differentiation of human hematopoietic stem/progenitor cells (HSPCs). The present study examined the effect of heavy ion beams on gene expression in human HSPCs, especially during early stage of megakaryocytopoiesis. Human CD34+ cells were exposed to monoenergetic carbon-ion beams (290 MeV/nucleon, LET = 50 KeV/um) that were generated by an accelerator (Heavy Ion Medical Accelerator in Chiba). The expression of various genes related to early hematopoiesis, megakaryocytopoiesis/erythropoiesis, cytokine receptors and oxidative stress were analyzed by real-time RT-PCR. Friend leukemia virus integration 1, an early hematopoiesis-related gene, showed significantly higher mRNA expression than the control at 6 hr after irradiation. In contrast, no significant differences were observed in almost all of the other early hematopoiesis-related genes, cytokine receptor-coded genes and megakaryocytopoiesis/erythropoiesis-differentiation pathway-related genes, respectively. An analysis of the response of the genes to oxidative stress revealed the expression of heme oxygenase 1 to show a 1.5-fold and 11.9-fold increase from the day 0 control at 24 hr after 0.5 Gy and 2 Gy irradiation, respectively. Similarly, the NAD(P)H dehydrogenase-quinone 1 expression also showed a 22.0-fold and a 21.8-fold increase at 6 hr in comparison to the initial control. These results showed that the heavy ion beams affect megakaryocytopoiesis/erythropoiesis differentiation of human HSPCs on the gene expression level.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1269/jrr.09058","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Monzen, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"453582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"453583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshino, Hironori"}],"nameIdentifiers":[{"nameIdentifier":"453584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Eguchi-Kasai, Kiyomi"}],"nameIdentifiers":[{"nameIdentifier":"453585","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Yoshinao"}],"nameIdentifiers":[{"nameIdentifier":"453586","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kashiwakura, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"453587","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"453588","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"門前 暁","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453589","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 賢次","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453590","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉野 浩教","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453591","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笠井 清美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453592","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"阿部 由直","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453593","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柏倉 幾郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"453594","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Heavy ion beam irradiation regulates the mRNA expression in megakaryocytopoiesis from human hematopoietic stem/progenitor cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Heavy ion beam irradiation regulates the mRNA expression in megakaryocytopoiesis from human hematopoietic stem/progenitor cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-28"},"publish_date":"2009-09-28","publish_status":"0","recid":"45645","relation_version_is_last":true,"title":["Heavy ion beam irradiation regulates the mRNA expression in megakaryocytopoiesis from human hematopoietic stem/progenitor cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:01:18.440603+00:00"}