{"created":"2023-05-15T14:35:23.349607+00:00","id":45560,"links":{},"metadata":{"_buckets":{"deposit":"4c71c8aa-4675-4135-94b0-8586e1b5f178"},"_deposit":{"created_by":1,"id":"45560","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45560"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045560","sets":["1"]},"author_link":["452719","452718","452721","452724","452723","452717","452725","452720","452722"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"426","bibliographicPageStart":"419","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The purpose of this study is to reveal characteristics of 64Cu-labeled diacetyl-bis(N4-methylthiosemicarbazone) ([64Cu]Cu-ATSM) during\ncell proliferation and hypoxia by autoradiography imaging and immunohistochemical staining.\nMethods: The intratumoral distributions of [64Cu]Cu-ATSM and [18F]-2-fluoro-2-deoxy-D-gloucose ([18F]FDG) in mice implanted with\nLewis lung carcinoma (LLC1) tumor cells according to dual autoradiography were compared with the immunohistochemical staining patterns\nof proliferating markers [Ki-67 and 5-bromo-2'-deoxyuridine (BrdU)] and a hypoxic marker (pimonidazole). A clonogenic assay was\nperformed using the cells of LLC1 tumor-implanted mice, and it was compared with the distribution of [64Cu]Cu-ATSM.\nResults: [64Cu]Cu-ATSM mainly accumulated at the edge of tumors, whereas [18F]FDG was distributed inside the tumor and inside the\n[64Cu]Cu-ATSM accumulation. The number of Ki-67-positive cells/area tended to increase with [18F]FDG accumulation and decrease with\n[64Cu]Cu-ATSM accumulation. On the other hand, the number of BrdU-positive cells/area was negatively correlated with [18F]FDG\naccumulation and positively correlated with [64Cu]Cu-ATSM accumulation. High [64Cu]Cu-ATSM accumulation was found outside the\nhigh-[18F]FDG-accumulation and pimonidazole-positive regions. Colony formation ability was significantly higher in the tumor cells\nobtained from high-[64Cu]Cu-ATSM-accumulation regions than the cells from the intermediate- and the low-accumulation regions.\nConclusion: [64Cu]Cu-ATSM accumulation regions in tumor cells indicate quiescent but clonogenic tumor cells under mild hypoxia. [64Cu]\nCu-ATSM could play an important role in planning appropriate tumor radiotherapy.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oh, Myungmi"}],"nameIdentifiers":[{"nameIdentifier":"452717","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"452718","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Masato"}],"nameIdentifiers":[{"nameIdentifier":"452719","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"452720","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"452721","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kudo, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"452722","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujieda, Shigeharu"}],"nameIdentifiers":[{"nameIdentifier":"452723","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"452724","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"452725","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Radio-copper-labeled Cu-ATSM: an indicator of quiescent but clonogenic cells under mild hypoxia in a Lewis lung carcinoma model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radio-copper-labeled Cu-ATSM: an indicator of quiescent but clonogenic cells under mild hypoxia in a Lewis lung carcinoma model"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-06-29"},"publish_date":"2009-06-29","publish_status":"0","recid":"45560","relation_version_is_last":true,"title":["Radio-copper-labeled Cu-ATSM: an indicator of quiescent but clonogenic cells under mild hypoxia in a Lewis lung carcinoma model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:02:16.599592+00:00"}