@article{oai:repo.qst.go.jp:00045529,
 author = {Lohith, Talakad and Furukawa, Takako and Mori, Tetsuya and Kobayashi, Masato and Fujibayashi, Yasuhisa and 古川 高子},
 issue = {5},
 journal = {Molecular Imaging and Biology},
 month = {Sep},
 note = {PURPOSE: The purpose of the study is to evaluate the feasibility of human estrogen receptor alpha ligand binding domain (hERL) as a reporter gene in combination with positron emission tomography (PET) probe, 16alpha-[18F]fluoro-17beta-estradiol (FES), in an adenovirus gene delivery system. METHODS: An adenoviral vector (test), carrying hERL gene and a model angiogenesis therapeutic gene (human thymidine phosphorylase, hTP) was constructed along with a control vector. In vitro radioligand binding and expression studies were performed on various cell lines. The control and test viruses were injected into contralateral adductor muscles of a rat followed by FES-PET imaging and immunohistochemical staining of resected muscle samples. RESULTS: A high FES uptake accompanied by hERL and hTP expression was obtained both in vitro and in vivo by the test adenovirus infection. CONCLUSION: Considering the versatile tissue permeability of the probe, highly efficient gene expression, and safeness for human use, we expect our reporter gene system to have favorable characteristics for clinical application.},
 pages = {245--252},
 title = {Basic evaluation of FES-hERL PET tracer-reporter gene system for in vivo monitoring of adenoviral-mediated gene therapy},
 volume = {10},
 year = {2008}
}