{"created":"2023-05-15T14:35:17.702599+00:00","id":45474,"links":{},"metadata":{"_buckets":{"deposit":"14b0b0bb-64e3-43e8-b95a-298d781dc0f2"},"_deposit":{"created_by":1,"id":"45474","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45474"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045474","sets":["1"]},"author_link":["451840","451846","451849","451852","451853","451847","451854","451842","451841","451839","451844","451848","451845","451843","451850","451851"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"255","bibliographicPageStart":"248","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"Journal of Trace Elements in Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background and aim: Molecular diagnostics and therapeutics of human mesothelioma using disease-related markers present major challenges in clinical practice. To identify biochemical alternations that would be markers of human mesothelioma, we measured the intracellular steady-state levels of biologically important trace metals such as manganese (Mn), copper (Cu), and zinc (Zn) in a human mesothelial cell line, MeT-5A, and in five human mesothelioma cell lines (MSTO-211H, NCI-H226, NCI-H2052, NCI-H2452, ACC-MESO-1) by inductively coupled plasma-mass spectrometry (ICP-MS). We also aimed to investigate whether the alterations were related to the\nintracellular status of metal-containing superoxide dismutase (SOD).\nResults: There were no significant differences in the contents of the trace metals among MeT-5A, MSTO-211H, and ACC-MESO-1 cells. However, each of the other three mesothelioma cell lines had a unique characteristic in terms of the intracellular amounts of the metals; NCI-H226 contained an extremely high level ofMn, an amount 7.3-fold higher than that in MeT-5A. NCI-H2052 had significantly higher amounts of Cu (3.4-fold) and Zn (1.3-fold) compared with MeT-5A. NCI-H2452 contained about 5.8-fold the amount of Cu and 2.5-fold that of Mn compared with MeT-5A. As for the intracellular levels of copper/zinc-SOD (Cu/Zn-SOD) and manganese-SOD (Mn-SOD), those of Cu/Zn-SOD were relatively unchanged among the cells tested, and no notable correlation with Cu or Zn contents was observed. On the other hand, all mesothelioma cells highly expressed Mn-SOD compared with MeT-5A, and a very high expression of the enzyme with a robust activity was observed in the two mesothelioma cells (NCI-H226, NCI-H2452) containing a large amount of Mn.\nConclusions: In comparison with MeT-5A human mesothelial cells, some human mesothelioma cells had significantly higher amounts of Mn or Cu and one mesothelioma cell had a significantly higher amount of Zn.\nInterestingly, all mesothelioma cells overexpressed Mn-SOD compared with MeT-5A, and the cells whose Mn-SOD activity was increased contained higher amounts of Mn. It seemed that intracellular Mn content was positively correlated with Mn-SOD, suggesting that the intracellular Mn level is associated with Mn-SOD activity. These\nbiochemical signatures could be potential disease-related markers of mesothelioma.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jtemb.2008.05.001","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"www.elsevier.de/jtemb"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"www.elsevier.de/jtemb","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0946-672X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Sumitaka"}],"nameIdentifiers":[{"nameIdentifier":"451839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koshikawa, Michiko"}],"nameIdentifiers":[{"nameIdentifier":"451840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Isao"}],"nameIdentifiers":[{"nameIdentifier":"451841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hachiya, Misao"}],"nameIdentifiers":[{"nameIdentifier":"451842","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"451843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akashi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"451844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"451845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"451846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川 純崇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451847","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"越川 道子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451848","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 功","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451849","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"蜂谷 みさを","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451850","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451851","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石 真言","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉田 聡","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451853","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"451854","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Alterations in manganese, copper, and zinc contents, and intracellular status of the metal-containing superoxide dismutase in human mesothelioma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Alterations in manganese, copper, and zinc contents, and intracellular status of the metal-containing superoxide dismutase in human mesothelioma cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-13"},"publish_date":"2009-04-13","publish_status":"0","recid":"45474","relation_version_is_last":true,"title":["Alterations in manganese, copper, and zinc contents, and intracellular status of the metal-containing superoxide dismutase in human mesothelioma cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:03:18.414946+00:00"}