{"created":"2023-05-15T14:35:06.269381+00:00","id":45301,"links":{},"metadata":{"_buckets":{"deposit":"df6c8b84-c75d-42bc-a0ee-ba9eb76b5514"},"_deposit":{"created_by":1,"id":"45301","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45301"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045301","sets":["1"]},"author_link":["450038","450039","450034","450036","450033","450035","450032","450037"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"611","bibliographicPageStart":"605","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Toxicology Mechanisms and Methods"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chronic ingestion of arsenic in polluted food and water can\ncause various human disorders including skin and lung cancers. Sensitive\nbiomarkers from human tissue/cells could help to prevent chronic intoxication\nwith low-dose arsenite. Using High-Coverage Expression Profiling (HiCEP),\nan Amplified Fragment Length Polymorphism (AFLP)-based gene expression\nprofiling technique, we analyzed the expression of approximately 11,000\ngenes in human lung fibroblasts (HFLIII) and compared the profiles between\ncells, treated and untreated with 1 uM sodium arsenite (NaAsO2). Hundreds\nof genes appeared upregulated and downregulated more two-fold 2 h after the\ntreatment. Marked induction was found (>4.4-fold) in a few genes including\nHMOX1, INHBA, and ANKRD11. Induction of the HMOX1 was detected\nwith a dose of arsenite at as low as 0.3 uM(0.04 ppm) and reached its maximum\nat 4 h after the treatment. The arsenite-induced HMOX1 expression was\nattenuated by the promoted glutathione (GSH) synthesis by N-acetyl-L-cysteine\n(NAC). However, it was not affected by pretreating the cells with general\nradical scavengers, consistent with the fact that ionizing radiation at either\nhigh- or low-doses has never induced HMOX1 in the same assay system. Thus,\ninduction of HMOX1 gene is highly sensitive and also selective against arsenite\nin the cells. The present process could provide a useful strategy for exploring\nbiomarkers that might help in assessing the known and unknown risks of any\nnatural and artificial toxic reagents.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1537-6516","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suetomi, Katsutoshi"}],"nameIdentifiers":[{"nameIdentifier":"450032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Sentaro"}],"nameIdentifiers":[{"nameIdentifier":"450033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"450034","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"450035","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"末冨 勝敏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"450036","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 千太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"450037","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保田 善久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"450038","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"450039","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of Genes Responding to Low-Dose Arsenite Using HiCEP","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of Genes Responding to Low-Dose Arsenite Using HiCEP"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-20"},"publish_date":"2008-10-20","publish_status":"0","recid":"45301","relation_version_is_last":true,"title":["Identification of Genes Responding to Low-Dose Arsenite Using HiCEP"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:05:21.111195+00:00"}