@article{oai:repo.qst.go.jp:00045301,
 author = {Suetomi, Katsutoshi and Takahashi, Sentaro and Kubota, Yoshihisa and Fujimori, Akira and 末冨 勝敏 and 高橋 千太郎 and 久保田 善久 and 藤森 亮},
 issue = {7},
 journal = {Toxicology Mechanisms and Methods},
 month = {Sep},
 note = {Chronic ingestion of arsenic in polluted food and water can
cause various human disorders including skin and lung cancers. Sensitive
biomarkers from human tissue/cells could help to prevent chronic intoxication
with low-dose arsenite. Using High-Coverage Expression Profiling (HiCEP),
an Amplified Fragment Length Polymorphism (AFLP)-based gene expression
profiling technique, we analyzed the expression of approximately 11,000
genes in human lung fibroblasts (HFLIII) and compared the profiles between
cells, treated and untreated with 1 uM sodium arsenite (NaAsO2). Hundreds
of genes appeared upregulated and downregulated more two-fold 2 h after the
treatment. Marked induction was found (>4.4-fold) in a few genes including
HMOX1, INHBA, and ANKRD11. Induction of the HMOX1 was detected
with a dose of arsenite at as low as 0.3 uM(0.04 ppm) and reached its maximum
at 4 h after the treatment. The arsenite-induced HMOX1 expression was
attenuated by the promoted glutathione (GSH) synthesis by N-acetyl-L-cysteine
(NAC). However, it was not affected by pretreating the cells with general
radical scavengers, consistent with the fact that ionizing radiation at either
high- or low-doses has never induced HMOX1 in the same assay system. Thus,
induction of HMOX1 gene is highly sensitive and also selective against arsenite
in the cells. The present process could provide a useful strategy for exploring
biomarkers that might help in assessing the known and unknown risks of any
natural and artificial toxic reagents.},
 pages = {605--611},
 title = {Identification of Genes Responding to Low-Dose Arsenite Using HiCEP},
 volume = {18},
 year = {2008}
}