{"created":"2023-05-15T14:35:05.814272+00:00","id":45294,"links":{},"metadata":{"_buckets":{"deposit":"356f907f-18ca-4426-b580-59c7da089971"},"_deposit":{"created_by":1,"id":"45294","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45294"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045294","sets":["1"]},"author_link":["449960","449962","449964","449967","449963","449961","449965","449966"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"787","bibliographicPageStart":"778","bibliographicVolumeNumber":"84","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: To isolate and to characterize cells expressing RNA polymerase II tagged with green fluorescent protein for analyses of the effects of ionizing radiation on transcription in living cells.\nMaterials and methods: We introduced an alpha-amanitin-resistant mutation into a vector encoding the largest subunit of RNA polymerase II tagged with green fluorescent protein (GFP-pol). Cell lines stably expressing functional GFP-pol were isolated under selection with alpha-amanitin from a Chinese hamster cell line, CHO-K1, and a radiation-sensitive mutant CHO cell line, XR-1.\nResults: We tested the functionality of the fusion protein in vivo by determining RNA synthesis activity by incorporation of nucleoside analogues. Both CHO-K1 and XR-1 cells expressing GFP-pol had properties similar to those of their respective parental cell lines, indicating that GFP-pol is functional.\nConclusions: These stable lines might prove useful for analyses of the roles of transcription after ionizing radiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0955-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hongou, Etsuko"}],"nameIdentifiers":[{"nameIdentifier":"449960","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishihara, Yoshie"}],"nameIdentifiers":[{"nameIdentifier":"449961","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugaya, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"449962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugaya, Kimihiko"}],"nameIdentifiers":[{"nameIdentifier":"449963","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"本郷 悦子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449964","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原 よし江","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449965","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅谷 恵子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449966","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅谷 公彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449967","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of cells expressing RNA polymerase II tagged with green fluorescent protein: Effect of ionizing irradiation on RNA synthesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of cells expressing RNA polymerase II tagged with green fluorescent protein: Effect of ionizing irradiation on RNA synthesis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-09"},"publish_date":"2008-10-09","publish_status":"0","recid":"45294","relation_version_is_last":true,"title":["Characterization of cells expressing RNA polymerase II tagged with green fluorescent protein: Effect of ionizing irradiation on RNA synthesis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:05:26.015168+00:00"}