@article{oai:repo.qst.go.jp:00045294,
 author = {Hongou, Etsuko and Ishihara, Yoshie and Sugaya, Keiko and Sugaya, Kimihiko and 本郷 悦子 and 石原 よし江 and 菅谷 恵子 and 菅谷 公彦},
 issue = {9},
 journal = {International Journal of Radiation Biology},
 month = {Sep},
 note = {Purpose: To isolate and to characterize cells expressing RNA polymerase II tagged with green fluorescent protein for analyses of the effects of ionizing radiation on transcription in living cells.
Materials and methods: We introduced an alpha-amanitin-resistant mutation into a vector encoding the largest subunit of RNA polymerase II tagged with green fluorescent protein (GFP-pol). Cell lines stably expressing functional GFP-pol were isolated under selection with alpha-amanitin from a Chinese hamster cell line, CHO-K1, and a radiation-sensitive mutant CHO cell line, XR-1.
Results: We tested the functionality of the fusion protein in vivo by determining RNA synthesis activity by incorporation of nucleoside analogues. Both CHO-K1 and XR-1 cells expressing GFP-pol had properties similar to those of their respective parental cell lines, indicating that GFP-pol is functional.
Conclusions: These stable lines might prove useful for analyses of the roles of transcription after ionizing radiation.},
 pages = {778--787},
 title = {Characterization of cells expressing RNA polymerase II tagged with green fluorescent protein: Effect of ionizing irradiation on RNA synthesis},
 volume = {84},
 year = {2008}
}