{"created":"2023-05-15T14:35:05.155530+00:00","id":45284,"links":{},"metadata":{"_buckets":{"deposit":"cf03ee59-923e-4fe9-bd6e-896ea98277e5"},"_deposit":{"created_by":1,"id":"45284","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45284"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045284","sets":["1"]},"author_link":["449856","449857","449849","449858","449853","449850","449847","449851","449855","449854","449848","449852","449860","449859"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"282","bibliographicPageStart":"269","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Genetica"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The W chromosome of the silkworm Bombyx mori is devoid of functional genes, except for the putative female-determining gene (Fem). To localize Fem, we investigated the presence of W-specific DNA markers on strains in which an autosomal fragment containing dominant marker genes was attached to the W chromosome. We produced new W-chromosomal fragments from the existing Zebra-W strain (T(W;3)Ze chromosome) by X-irradiation, and then carried out deletion mapping of these and sex-limited yellow cocoon strains (T(W;2)Y-Chu, -Abe and -Ban types) from different Japanese stock centers. Of 12 RAPD markers identified in the normal W chromosomes of most silkworm strains in Japan, the newly irradiated W(B-YL-YS)Ze chromosome contained three, the T(W;2)Y-Chu chromosome contained six, and the T(W;2)Y-Abe and -Ban chromosomes contained only one (W-Rikishi). To investigate the ability of the reduced W-chromosome translocation fragments to form heterochromatin bodies, which are found in nuclei of normal adult female sucking stomachs, we examined cells of the normal type p50 strain and the T(W;2)Y-Chu and -Abe strains. A single sex heterochromatin body was found in nuclei of p50 females, whereas we detected only small sex heterochromatin bodies in the T(W;2)Y-Chu strain and no sex heterochromatin body in the T(W;2)Y-Abe strain. Since adult females of all strains were normal and fertile, we conclude that only extremely limited region, containing the W-Rikishi RAPD sequence of the W chromosome, is required to determine femaleness. Based on a comparison of the normal W-chromosome and 7 translocation and W-deletion strains we present a map of Fem relative to the 12 W-specific RAPD markers.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0016-6707","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abe, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"449847","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujii, T"}],"nameIdentifiers":[{"nameIdentifier":"449848","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoyama, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"449849","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kakehashi, Hirokazu"}],"nameIdentifiers":[{"nameIdentifier":"449850","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ajimura, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"449851","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mita, Kazuei"}],"nameIdentifiers":[{"nameIdentifier":"449852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"449853","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"449854","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Toru"}],"nameIdentifiers":[{"nameIdentifier":"449855","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"449856","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"味村 正博","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449857","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田 和英","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449858","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449859","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 剛弘","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449860","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of the female-determining region of the W chromosome in Bombyx mori","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of the female-determining region of the W chromosome in Bombyx mori"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-08"},"publish_date":"2008-10-08","publish_status":"0","recid":"45284","relation_version_is_last":true,"title":["Identification of the female-determining region of the W chromosome in Bombyx mori"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:05:33.381252+00:00"}