@article{oai:repo.qst.go.jp:00045242,
 author = {Iwakawa, Mayumi and Hamada, Nobuyuki and Imadome, Kaori and Funayama, Tomoo and Sakashita, Tetsuya and Kobayashi, Yasuhiko and Imai, Takashi and 岩川 眞由美 and 浜田 信行 and 今留 香織 and 舟山 知夫 and 坂下 哲哉 and 小林 泰彦 and 今井 高志},
 issue = {1-2},
 journal = {Fundamental and Molecular Mechanisms of Mutagenesis : A Section of Mutation Research},
 month = {Jul},
 note = {Evidence has accumulated that ionizing radiation induces biological effects in non-irradiated bystander cells having received signals from directly irradiated cells; however, energetic heavy ion-induced bystander response is incompletely characterized. Here we performed microarray analysis of irradiated and bystander fibroblasts in confluent cultures. To see the effects in bystander cells, each of 1, 5 and 25 sites was targeted with 10 particles of carbon ions (18.3MeV/u, 103keV/mum) using microbeams, where particles traversed 0.00026, 0.0013 and 0.0066% of cells, respectively. diated cells, cultures were exposed to 10% survival dose (D), 0.1D and 0.01D of corresponding broadbeams (108keV/mum). Irrespective of the target numbers (1, 5 or 25 sites) and the time (2 or 6h postirradiation), similar expression changes were observed in bystander cells. Among 874 probes that showed more than 1.5-fold changes in bystander cells, 25% were upregulated and the remainder downregulated. These included genes related to cell communication (PIK3C2A, GNA13, FN1, ANXA1 and IL1RAP), stress response (RAD23B, ATF4 and EIF2AK4) and cell cycle (MYCN, RBBP4 and NEUROG1). Pathway analysis revealed serial bystander activation of G protein/PI-3 kinase pathways. Instead, genes related to cell cycle or death (CDKN1A, GADD45A, NOTCH1 and BCL2L1), and cell communication (IL1B, TCF7 and ID1) were upregulated in irradiated cells, but not in bystander cells. Our results indicate different expression profiles in irradiated and bystander cells, and imply that intercellular signaling between irradiated and bystander cells activate intracellular signaling, leading to the transcriptional stress response in bystander cells.},
 pages = {57--67},
 title = {Expression profiles are different in carbon ion-irradiated normal human fibroblasts and their bystander cells},
 volume = {642},
 year = {2008}
}