{"created":"2023-05-15T14:35:01.316093+00:00","id":45230,"links":{},"metadata":{"_buckets":{"deposit":"f7ee2f76-99bd-43c0-b4a0-fbce5dbd74ba"},"_deposit":{"created_by":1,"id":"45230","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45230"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045230","sets":["1"]},"author_link":["449272","449267","449275","449278","449268","449280","449273","449274","449277","449279","449270","449271","449276","449269","449266"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"122","bibliographicPageStart":"117","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"Carcinogenesis"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The DNA double-strand breaks (DSBs) repair pathway has been implicated in maintaining genomic integrity via suppression of chromosomal rearrangements. DNA-dependent protein kinase (DNA-PK) has an important role with DNA Dabs repair.   In this study, 93 of untreated cancer patients and 41 of cancer-free healthy volunteers were enrolled.   Peripheral blood was collected, separated and centrifuged; DNA-PK activity was measured by DNA-pull-down assay.   The expression of DNA-PKcs, Ku70 and Ku86 were examined by RT-PCR assay and western blotting.   Chromosomal aberrations were examined by cytogenetic methods. DNA-PK activities of peripheral blood lymphocytes (PBL) in patients with uterine cervix or breast cancer were significantly lower than those in normal volunteers. Age and smoking had no association with DNA-PK activity, whereas DNA-PK activity and the expression of Ku70, Ku86 and DNA-PKcs in RT-PCR were interrelated.   A similar tendency was seen in western blot assay but less clear than in RT-PCR. Therefore, the association between DNA-PK activity and expression of DNA-PK in protein level could not be concluded.   The frequency of chromosome aberration, such as dicentric chromosomes and excess fragment increased as the DNA-PK activity decreased.  In conclusion, DNA-PK activity is associated with chromosomal instability.   DNA-PK activity in PBL is associated with risk of breast and uterine cervix cancer.  DNA-PK activity in PBL can be used to select individuals for whom an examination should be performed because of their increased susceptibility to breast and uterine cervix cancer.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0143-3334","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Someya, Masanori"}],"nameIdentifiers":[{"nameIdentifier":"449266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakata, Koh-ichi"}],"nameIdentifiers":[{"nameIdentifier":"449267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"449268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"449269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Monobe, Manami"}],"nameIdentifiers":[{"nameIdentifier":"449270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikeda, Hideyuki"}],"nameIdentifiers":[{"nameIdentifier":"449271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"449272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hosoi, Yoshio"}],"nameIdentifiers":[{"nameIdentifier":"449273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Norio"}],"nameIdentifiers":[{"nameIdentifier":"449274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hareyama, Masato"}],"nameIdentifiers":[{"nameIdentifier":"449275","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 義久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449276","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"物部 真奈美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安藤 興一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449278","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"細井 義夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 紀夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449280","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The association of DNA-dependent protein kinase activity with chromosomal instability and risk of cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The association of DNA-dependent protein kinase activity with chromosomal instability and risk of cancer"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-07-23"},"publish_date":"2008-07-23","publish_status":"0","recid":"45230","relation_version_is_last":true,"title":["The association of DNA-dependent protein kinase activity with chromosomal instability and risk of cancer"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:06:11.877752+00:00"}