@article{oai:repo.qst.go.jp:00045230,
 author = {Someya, Masanori and Sakata, Koh-ichi and Matsumoto, Yoshihisa and Yamamoto, Hiroyuki and Monobe, Manami and Ikeda, Hideyuki and Ando, Koichi and Hosoi, Yoshio and Suzuki, Norio and Hareyama, Masato and 松本 義久 and 物部 真奈美 and 安藤 興一 and 細井 義夫 and 鈴木 紀夫},
 issue = {1},
 journal = {Carcinogenesis},
 month = {Jan},
 note = {The DNA double-strand breaks (DSBs) repair pathway has been implicated in maintaining genomic integrity via suppression of chromosomal rearrangements. DNA-dependent protein kinase (DNA-PK) has an important role with DNA Dabs repair.   In this study, 93 of untreated cancer patients and 41 of cancer-free healthy volunteers were enrolled.   Peripheral blood was collected, separated and centrifuged; DNA-PK activity was measured by DNA-pull-down assay.   The expression of DNA-PKcs, Ku70 and Ku86 were examined by RT-PCR assay and western blotting.   Chromosomal aberrations were examined by cytogenetic methods. DNA-PK activities of peripheral blood lymphocytes (PBL) in patients with uterine cervix or breast cancer were significantly lower than those in normal volunteers. Age and smoking had no association with DNA-PK activity, whereas DNA-PK activity and the expression of Ku70, Ku86 and DNA-PKcs in RT-PCR were interrelated.   A similar tendency was seen in western blot assay but less clear than in RT-PCR. Therefore, the association between DNA-PK activity and expression of DNA-PK in protein level could not be concluded.   The frequency of chromosome aberration, such as dicentric chromosomes and excess fragment increased as the DNA-PK activity decreased.  In conclusion, DNA-PK activity is associated with chromosomal instability.   DNA-PK activity in PBL is associated with risk of breast and uterine cervix cancer.  DNA-PK activity in PBL can be used to select individuals for whom an examination should be performed because of their increased susceptibility to breast and uterine cervix cancer.},
 pages = {117--122},
 title = {The association of DNA-dependent protein kinase activity with chromosomal instability and risk of cancer},
 volume = {27},
 year = {2006}
}