{"created":"2023-05-15T14:35:00.263487+00:00","id":45214,"links":{},"metadata":{"_buckets":{"deposit":"e8a69071-c51b-45c0-bb8d-202c0f0013fd"},"_deposit":{"created_by":1,"id":"45214","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45214"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045214","sets":["1"]},"author_link":["1011653","1011649","1011652","1011650","1011651"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"571","bibliographicPageStart":"563","bibliographicVolumeNumber":"167","bibliographic_titles":[{"bibliographic_title":"Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To clarify the relationship between CDC2 kinase activity and radiation-induced apoptosis, we examined whether the cyclin-dependent kinase (CDK) inhibitor purvalanol A enhanced radiation-induced apoptosis in gastric tumor cells. MKN45 cells exposed to 20 Gy of X rays increased the CDC2 kinase activity and the expression of regulatory proteins (phospho-CDC2 and cyclin B1) of the G2/M phase, followed by activation of the G2/M checkpoint, whereas the treatment of X-irradiated MKN45 cells with 20 microM purvalanol A suppressed the increase in the CDC2 kinase activity and expression of the G2/M-phase regulatory proteins and reduced the fraction of the cells in the G2/M phase in the cell cycle. Furthermore, this treatment resulted in not only a significant increase in radiation-induced apoptosis but also the loss of clonogenicity in both MKN45 (p53-wild) and MKN28 (p53-mutated) cells. The expression of anti-apoptosis proteins, inhibitor of apoptosis protein (IAP) family members (survivin and XIAP) and BCL2 family members (Bcl-X(L) and Bcl-2), in purvalanol A-treated cells with and without X rays was significantly lower than for cells exposed to X rays alone. These results suggest that the inhibition of radiation-induced CDC2 kinase activity by purvalanol A induces apoptosis through the enhancement of active fragments of caspase 3.","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"17474786","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1667/rr0622.1","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0033-7587","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Iizuka, Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"1011649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inanami, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"1011650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kashiwakura, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"1011651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuwabara, Mikinori"}],"nameIdentifiers":[{"nameIdentifier":"1011652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daisuke, Iizuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1011653","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-04"},"publish_date":"2007-12-04","publish_status":"0","recid":"45214","relation_version_is_last":true,"title":["Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:59:12.823582+00:00"}