{"created":"2023-05-15T14:34:58.493999+00:00","id":45187,"links":{},"metadata":{"_buckets":{"deposit":"ec4e7552-cf08-4952-a3a0-1573980cf483"},"_deposit":{"created_by":1,"id":"45187","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45187"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045187","sets":["1"]},"author_link":["448831","448833","448832","448829","448830"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"20","bibliographicPageEnd":"2954","bibliographicPageStart":"2950","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Oncogene"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The t(2;5) chromosomal translocation occurs in anaplastic large-cell lymphoma arising from activated T lymphocytes. This genomic rearrangement generates the nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) oncoprotein that is a chimeric protein consisting of parts of the nuclear protein NPM and ALK receptor protein-tyrosine kinase. We used yeast two-hybrid screening to identify an adaptor protein Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target (SNT)-2 as a new partner that interacted with the cytoplasmic domain of ALK. Immunoprecipitation assay revealed that SNT-1 and SNT-2 interacted with NPM-ALK and kinase-negative NPM-ALK mutant. Y156, Y567 and a 19-amino-acid sequence (aa 631-649) of NPM-ALK were essential for this interaction. The interaction through Y156 and Y567 was dependent on phosphorylation of these tyrosines, whereas the interaction through the 19-amino-acid sequence was independent of phosphorylation. NPM-ALK mutant protein mutated at these three binding sites showed significantly reduced transforming activity. This transformation-defective NPM-ALK mutant still interacted with signal transducing proteins such as phospholipase C-gamma and phosphatidylinositol 3-kinase, which were previously reported to be relevant to NPM-ALK-dependent tumorigenesis. These observations indicate that the three SNT-binding sites of NPM-ALK are important for its transforming activity. This raises a possibility that SNT family proteins play significant roles in cellular transformation triggered by NPM-ALK, which though remains to be verified.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/sj.onc.1210095","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0950-9232","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chikamori, Minoru"}],"nameIdentifiers":[{"nameIdentifier":"448829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimoto, Jiro"}],"nameIdentifiers":[{"nameIdentifier":"448830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishizumi, Noriko"}],"nameIdentifiers":[{"nameIdentifier":"448831","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"448832","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"近森 穣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448833","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of multiple SNT-binding sites on NPM-ALK oncoprotein and their involvement in cell transformation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of multiple SNT-binding sites on NPM-ALK oncoprotein and their involvement in cell transformation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-06-11"},"publish_date":"2008-06-11","publish_status":"0","recid":"45187","relation_version_is_last":true,"title":["Identification of multiple SNT-binding sites on NPM-ALK oncoprotein and their involvement in cell transformation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:06:38.526076+00:00"}