@article{oai:repo.qst.go.jp:00045182, author = {Takabatake, Takashi and Ishihara, Hiroshi and Ohmachi, Yasushi and Tanaka, Izumi and M., Nakamura Masako and Fujikawa, Katsuyoshi and Hirouchi, Tokuhisa and Kakinuma, Shizuko and Shimada, Yoshiya and Oghiso, Yoichi and Tanaka, Kimio and 高畠 貴志 and 石原 弘 and 大町 康 and 田中 泉 and 柿沼 志津子 and 島田 義也 and 田中 公夫}, issue = {10}, journal = {Nucleic Acids Research}, month = {May}, note = {Mammalian genomes contain numerous evolutionary harbored mobile elements, a part of which are still active and may cause genomic instability. Their movement and positional diversity occasionally result in phenotypic changes and variation by causing altered expression or disruption of neighboring host genes. Here, we describe a novel microarraybased method by which dispersed genomic locations of a type of retrotransposon in a mammalian genome can be identified. Using this method, we mapped the DNA elements for a mouse retrotransposon, intracisternal A-particle (IAP), within genomes of C3H/He and C57BL/6J inbred mouse strains; consequently we detected hundreds of probable IAP cDNA–integrated genomic regions, in which a considerable number of strain-specific putative insertions were included. In addition, by comparing genomic DNAs from radiation-induced myeloid leukemia cells and its reference normal tissue, we detected three genomic regions around which an IAP element was integrated. These results demonstrate the first successful genome-wide mapping of a retrotransposon type in a mammalian genome.}, pages = {e59-1--e59-11}, title = {Microarray-based global mapping of integration sites for the retrotransposon, intracisternal A-particle, in the mouse genome}, volume = {36}, year = {2008} }