{"created":"2023-05-15T14:34:54.594264+00:00","id":45128,"links":{},"metadata":{"_buckets":{"deposit":"f733081d-bcc9-4ea3-83e8-e2e2d3eb3ddd"},"_deposit":{"created_by":1,"id":"45128","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45128"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045128","sets":["1"]},"author_link":["448149","448147","448150","448146","448148"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"61","bibliographicPageStart":"54","bibliographicVolumeNumber":"365","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study accents on the relationship between dicing, nuclease stability, and RNAi activity of various types of chemically modified symmetric and asymmetric dsRNAs, covalently bound with amino-groups or cholesterol at one or both terminals. All modified dsRNAs were subjected to cleavage by recombinant Dicer enzyme. They possessed a high resistance to nuclease degradation in cell cultured medium and an excellent RNAi activity in viable cells. The best stability and RNAi activity was detected for 5' -sense amino-modified RNAs. These modifications manifested also a high long-term gene silencing effect within seven days post-transfection, while the RNAi activity of the native 21nt siRNA expired within two days. The conjugation of dsRNA with cholesterol at 5' -sense end resulted in easy intracellular delivery without transfection reagents. After a direct transfection in cells, the cholesterol-conjugated 27nt dsRNA possessed a higher RNAi activity than cholesterol-conjugated 21nt siRNA.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2007.10.116","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kubo, Takanori"}],"nameIdentifiers":[{"nameIdentifier":"448146","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhelev, Zhivko"}],"nameIdentifiers":[{"nameIdentifier":"448147","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohba, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"448148","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bakalova-Zheleva, Rumiana"}],"nameIdentifiers":[{"nameIdentifier":"448149","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"バカロバ ルミアナ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448150","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chemically modified symmetric and asymmetric duplex RNAs: An enhanced stability to nuclease degradation and gene silencing effect","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chemically modified symmetric and asymmetric duplex RNAs: An enhanced stability to nuclease degradation and gene silencing effect"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-03-25"},"publish_date":"2008-03-25","publish_status":"0","recid":"45128","relation_version_is_last":true,"title":["Chemically modified symmetric and asymmetric duplex RNAs: An enhanced stability to nuclease degradation and gene silencing effect"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:07:21.124674+00:00"}