@article{oai:repo.qst.go.jp:00045128,
 author = {Kubo, Takanori and Zhelev, Zhivko and Ohba, Hideki and Bakalova-Zheleva, Rumiana and バカロバ ルミアナ},
 issue = {1},
 journal = {Biochemical and Biophysical Research Communications},
 month = {Oct},
 note = {The present study accents on the relationship between dicing, nuclease stability, and RNAi activity of various types of chemically modified symmetric and asymmetric dsRNAs, covalently bound with amino-groups or cholesterol at one or both terminals. All modified dsRNAs were subjected to cleavage by recombinant Dicer enzyme. They possessed a high resistance to nuclease degradation in cell cultured medium and an excellent RNAi activity in viable cells. The best stability and RNAi activity was detected for 5' -sense amino-modified RNAs. These modifications manifested also a high long-term gene silencing effect within seven days post-transfection, while the RNAi activity of the native 21nt siRNA expired within two days. The conjugation of dsRNA with cholesterol at 5' -sense end resulted in easy intracellular delivery without transfection reagents. After a direct transfection in cells, the cholesterol-conjugated 27nt dsRNA possessed a higher RNAi activity than cholesterol-conjugated 21nt siRNA.},
 pages = {54--61},
 title = {Chemically modified symmetric and asymmetric duplex RNAs: An enhanced stability to nuclease degradation and gene silencing effect},
 volume = {365},
 year = {2007}
}