{"created":"2023-05-15T14:34:49.818189+00:00","id":45027,"links":{},"metadata":{"_buckets":{"deposit":"8ddbbb78-5aa5-4a54-8c10-0259d441e90e"},"_deposit":{"created_by":1,"id":"45027","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45027"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045027","sets":["1"]},"author_link":["447145","447146","447147","447150","447149","447148"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"6","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"The Journal of Dermatology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The skin is an external organ that is most frequently exposed to radiation. It is important to elucidate the influence\nof radiation exposure on the skin at the molecular level. To identify radiation-responsive genes in human skin cells,\nwe used microarray technology to examine the effects of irradiation on 641 genes in normal human epidermal\nkeratinocytes at 4 h and 8 h postirradiation with a cytotoxic dose of X-ray (10 Gy). We found that 18 genes were\nupregulated and 35 genes were downregulated in keratinocytes at 4 h and/or 8 h postirradiation. Ninjurin, whose\nfunction remains unknown in keratinocytes, was induced most strongly by X-irradiation. Several known apoptosisrelated\ngenes, such as TSC22, were also upregulated. We characterized Ninjurin and TSC22 induction after Xirradiation\nof normal human skin cells. The induction of the expression of Ninjurin and TSC22 mRNA in keratinocytes\nfollowing high-dose X-irradiation was confirmed by northern blot analysis. In dermal fibroblasts, Ninjurin, but not\nTSC22, was induced after X-ray irradiation. The dependence of both gene expression on the status of an\napoptosis regulator, p53, was found. In addition, the expression of both mRNA was induced upon treatment with\nan apoptosis inducer, etoposide. On the other hand, TSC22, but not Ninjurin, was induced and accumulated in\nkeratinocytes upon treatment with an apoptosis inducer, anisomycin. However, in transient expression assay,\nEYFP-TSC22, as well as EYFP-Ninjurin or EYFP alone, did not induce apoptosis in keratinocytes in contrast\nto EYFP-GADD45. Taken together, these findings have important implications on the understanding of the\nmechanism underlying the complex response of skin cells following X-irradiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-2407","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koike, Manabu"}],"nameIdentifiers":[{"nameIdentifier":"447145","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ninomiya, Yasuharu"}],"nameIdentifiers":[{"nameIdentifier":"447146","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Aki"}],"nameIdentifiers":[{"nameIdentifier":"447147","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 学","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"447148","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮 康晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"447149","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 亜紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"447150","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Ninjurin and TSC22 induction after x-irradiation of normal human skin cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Ninjurin and TSC22 induction after x-irradiation of normal human skin cells."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-11-21"},"publish_date":"2007-11-21","publish_status":"0","recid":"45027","relation_version_is_last":true,"title":["Characterization of Ninjurin and TSC22 induction after x-irradiation of normal human skin cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:08:32.693683+00:00"}