@article{oai:repo.qst.go.jp:00045027,
 author = {Koike, Manabu and Ninomiya, Yasuharu and Koike, Aki and 小池 学 and 二宮 康晴 and 小池 亜紀},
 issue = {1},
 journal = {The Journal of Dermatology},
 month = {Jan},
 note = {The skin is an external organ that is most frequently exposed to radiation. It is important to elucidate the influence
of radiation exposure on the skin at the molecular level. To identify radiation-responsive genes in human skin cells,
we used microarray technology to examine the effects of irradiation on 641 genes in normal human epidermal
keratinocytes at 4 h and 8 h postirradiation with a cytotoxic dose of X-ray (10 Gy). We found that 18 genes were
upregulated and 35 genes were downregulated in keratinocytes at 4 h and/or 8 h postirradiation. Ninjurin, whose
function remains unknown in keratinocytes, was induced most strongly by X-irradiation. Several known apoptosisrelated
genes, such as TSC22, were also upregulated. We characterized Ninjurin and TSC22 induction after Xirradiation
of normal human skin cells. The induction of the expression of Ninjurin and TSC22 mRNA in keratinocytes
following high-dose X-irradiation was confirmed by northern blot analysis. In dermal fibroblasts, Ninjurin, but not
TSC22, was induced after X-ray irradiation. The dependence of both gene expression on the status of an
apoptosis regulator, p53, was found. In addition, the expression of both mRNA was induced upon treatment with
an apoptosis inducer, etoposide. On the other hand, TSC22, but not Ninjurin, was induced and accumulated in
keratinocytes upon treatment with an apoptosis inducer, anisomycin. However, in transient expression assay,
EYFP-TSC22, as well as EYFP-Ninjurin or EYFP alone, did not induce apoptosis in keratinocytes in contrast
to EYFP-GADD45. Taken together, these findings have important implications on the understanding of the
mechanism underlying the complex response of skin cells following X-irradiation.},
 pages = {6--17},
 title = {Characterization of Ninjurin and TSC22 induction after x-irradiation of normal human skin cells.},
 volume = {35},
 year = {2008}
}