{"created":"2023-05-15T14:34:45.991917+00:00","id":44939,"links":{},"metadata":{"_buckets":{"deposit":"cc384751-89f2-4dd2-9533-cce6e3664fdd"},"_deposit":{"created_by":1,"id":"44939","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44939"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044939","sets":["1"]},"author_link":["446288","446284","446289","446286","446291","446290","446287","446285"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"48","bibliographicPageStart":"41","bibliographicVolumeNumber":"142","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"N-beta-Alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an insect-induced antibacterial peptide, generates hydrogen peroxyde (H2O2) that exerts antitumour activity.  We have investigated the precise mechanism of H2O2 production from 5-S-GAD by autoxidation aiming to understand its action toward tumour cells.  Using the electron spin resonance (ESR) technique, we detected a strong signal due to radical formation from 5-S-GAD.  Surprisingly, the ESR signal of the radicalo derived from 5-S-GAD appeared after incubation for 30 min at 310 K in the buffer at pH 7.4; the signal was persistently detected for 10 h in the absence of catalytic metal ions.  The computer simulation of the observed ESR spectrum together with the theoretical calculation of the spin density of the radical species indicates that an o-semiquinone radical anion was formed from 5-S-GAD.  We demonstrated that H2O2 is produced via the formation of superoxide anion by the electron-transfer reduction of molecular oxygen by the 5-S-GAD anion, which is in equiliblium with 5-S-GAD in the aqueous solution.  The radical formation and the subsequent H2O2 production were inhibited by superoxide dismutase (SOD), when the antitumour activity of 5-S-GAD was inhibited by SOD.  Thus, the formation of the o-semiquinone radical anion would be necessary for the antitumour activity of 5-S-GAD as an intermediate in the production of cytotoxic H2O2.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Akiyama, Nobuko"}],"nameIdentifiers":[{"nameIdentifier":"446284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"446285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohkubo, Kei"}],"nameIdentifiers":[{"nameIdentifier":"446286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukuzumi, Shunichi"}],"nameIdentifiers":[{"nameIdentifier":"446287","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ozawa, Toshihiko"}],"nameIdentifiers":[{"nameIdentifier":"446288","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"446289","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中西 郁夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446290","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小澤 俊彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446291","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Long-Lived o-Semiquinone Radical Anion is Formed from N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an Insect-derived Antibacterial Substance","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Long-Lived o-Semiquinone Radical Anion is Formed from N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an Insect-derived Antibacterial Substance"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-08-14"},"publish_date":"2007-08-14","publish_status":"0","recid":"44939","relation_version_is_last":true,"title":["A Long-Lived o-Semiquinone Radical Anion is Formed from N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an Insect-derived Antibacterial Substance"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:09:33.931492+00:00"}