{"created":"2023-05-15T14:34:45.166060+00:00","id":44920,"links":{},"metadata":{"_buckets":{"deposit":"f0edbc59-3331-461f-a28e-3e00a9c2a7aa"},"_deposit":{"created_by":1,"id":"44920","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44920"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044920","sets":["1"]},"author_link":["446142","446140","446143","446141"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2675","bibliographicPageStart":"2669","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We describe an organotypic model of mouse skin consisting of a stratified sheet of epidermal keratinocytes and dermal fibroblasts within a contracted collagen gel. The model was designed to maintain the polarity of stratified keratinocytes and permit their long-term culture at an air-liquid interface. After air exposure, the thickness of the keratinocyte sheet transiently increased and then decreased to two cell layers at 2 weeks. The two-cell-layer structure is similar to that of the adult mouse epidermis. Cytokeratin 5 was localized in the lowest cell layer in the epithelial sheet, but cytokeratin 1 and loricrin were localized in the outer cell layers, resembling mouse skin. The expressions of interleukin 1alpha and 1beta in the keratinocytes and of keratinocyte growth factor 1 and 2 in the fibroblasts correlated with keratinocyte stratification. The mouse organotypic coculture is useful in studying epithelial cell-mesenchymal cell interactions in vitro.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1271/bbb.60266","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ikuta, Shuzo"}],"nameIdentifiers":[{"nameIdentifier":"446140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Yuriko"}],"nameIdentifiers":[{"nameIdentifier":"446141","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"446142","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 有里子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"446143","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mouse epidermal keratinocytes in three-dimensional organotypic coculture with dermal fibroblasts form a stratified sheet resembling skin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mouse epidermal keratinocytes in three-dimensional organotypic coculture with dermal fibroblasts form a stratified sheet resembling skin"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-23"},"publish_date":"2007-07-23","publish_status":"0","recid":"44920","relation_version_is_last":true,"title":["Mouse epidermal keratinocytes in three-dimensional organotypic coculture with dermal fibroblasts form a stratified sheet resembling skin"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:09:47.211243+00:00"}