@article{oai:repo.qst.go.jp:00044837,
 author = {Suzuki, Keiko and Tanaka, Izumi and Nakanishi, Ikuo and Kurematsu, Ayako and Yakumaru, Haruko and Ikota, Nobuo and Ishihara, Hiroshi and 鈴木 桂子 and 田中 泉 and 中西 郁夫 and 槫松 文子 and 薬丸 晴子 and 伊古田 暢夫 and 石原 弘},
 issue = {3-4},
 journal = {BioFactors},
 month = {},
 note = {Among antioxidative polyphenols, caffeic acid esters such as caffeic acid phenethyl ester (CAPE) and chlorogenic acid are contained in propolis, vegetables and coffee.  In this study, we compared the efficacy of some polyphenols on the activation level of a cytoprotective heme oxygenase-1 (HO-1) gene in RAW264.7 mouse macrophage cells using quantitative real-time RT-PCR.  The quantitative study revealed a variety of activation level of HO-1 gene by the chemicals.  CAPE and caffeic acid ethyl ester (CAEE) at the final concentration of 2 M drastically activated the HO-1 gene to 39.2-fold and 20.1-fold, respectively.  Curcumin, structurally related with caffeic acid and an element of turmeric, induced the HO-1 gene to 5.8-fold.  In contrast, no activation was observed by other caffeic acid esters such as chlorogenic acid and rosmarinic acid,.  Higher concentrations were necessary for the activation by an the antioxidant cysteamine and an electrophile diethyl maleate.  Although the inducible activities of CAPE and chlorogenic acid were distinctly different, they showed similar reductive capacities when determined by cyclic voltammetry.  These results show that the drastic activation of HO-1 gene by CAPE and CAEE is dependent upon their chemical structures, rather than the reductive activity of polyphenols, possibly reflecting the physiological effects of the nutritional elements.},
 pages = {151--158},
 title = {Drastic effect of several caffeic acid derivatives on the induction of heme oxygenase-1 expression revealed by quantitative real-time RT-PCR.},
 volume = {28},
 year = {2006}
}