{"created":"2023-05-15T14:34:34.120524+00:00","id":44669,"links":{},"metadata":{"_buckets":{"deposit":"e81ad939-1ccf-4deb-8741-f7f4c0fae234"},"_deposit":{"created_by":1,"id":"44669","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44669"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044669","sets":["1"]},"author_link":["443570","443571","443572","443569"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"3075","bibliographicPageStart":"3063","bibliographicVolumeNumber":"273","bibliographic_titles":[{"bibliographic_title":"The FEBS Journal"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The occurrence of DNA double strand breaks (DSBs) in the nucleus provokes in\nits structural organization a large-scale alteration whose molecular basis\nis still mostly unclear.  Here, we show that DSBs trigger preferential\nassembly of nucleoproteins in human cellular fractions and that they mediate\nthe separation of large protein-DNA aggregates from aqueous solution.  The\ninteraction among the aggregative nucleoproteins presents a dynamic\ncondition that allows the effective interaction of nucleoproteins with\nexternal molecules like free ATP and facilitates intrinsic DNA end-joining\nactivity.  This aggregative organization is functionally coacervate-like.\nThe key component is DNA-dependent protein kinase, DNA-PK, which can be\ncharacterized as a DNA-specific aggregation factor as well as a nuclear\nscaffold/matrix-interactive factor.  In the context of aggregation, the\nkinase activity of DNA-PK is essential for efficient DNA end-joining.  The\nmassive and functional concentration of nucleoproteins on DNA in vitro may\nrepresent a possible status of nuclear dynamics in vivo, which probably\nincludes the DNA-PK-dependent response to multiple DSBs.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1742-464X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahagi, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"443569","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsumi, Kouichi"}],"nameIdentifiers":[{"nameIdentifier":"443570","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高萩 真彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"443571","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"443572","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-10-13"},"publish_date":"2006-10-13","publish_status":"0","recid":"44669","relation_version_is_last":true,"title":["Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:12:44.467738+00:00"}