{"created":"2023-05-15T14:34:31.283800+00:00","id":44605,"links":{},"metadata":{"_buckets":{"deposit":"b0e0bf32-0711-4b65-b658-d75ed0557cb4"},"_deposit":{"created_by":1,"id":"44605","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44605"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044605","sets":["1"]},"author_link":["442971","442967","442969","442963","442962","442970","442972","442964","442968","442973","442965","442966"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"67","bibliographicPageStart":"59","bibliographicVolumeNumber":"165","bibliographic_titles":[{"bibliographic_title":"Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human and rodent cells proficient and deficient in non-homologous end joining (NHEJ) were irradiated with X rays, 70 keV/mum carbon ions, and 200 keV/mum iron ions, and the biological effects on these cells were compared. For wild-type CHO and normal human fibroblast (HFL III) cells, exposure to iron ions yielded the lowest cell survival, followed by carbon ions and then X rays. NHEJ-deficient xrs6 (a Ku80 mutant of CHO) and 180BR human fibroblast (DNA ligase IV mutant) cells showed similar cell survival for X and carbon-ion irradiation (RBE = approximately 1.0). This phenotype is likely to result from a defective NHEJ protein because xrs6-hamKu80 cells (xrs6 cells corrected with the wild-type KU80 gene) exhibited the wild-type response. At doses higher than 1 Gy, NHEJ-defective cells showed a lower level of survival with iron ions than with carbon ions or X rays, possibly due to inactivation of a radioresistant subpopulation. The G(1) premature chromosome condensation (PCC) assay with HFL III cells revealed LET-dependent impairment of repair of chromosome breaks. Additionally, iron-ion radiation induced non-repairable chromosome breaks not observed with carbon ions or X rays. PCC studies with 180BR cells indicated that the repair kinetics after exposure to carbon and iron ions behaved similarly for the first 6 h, but after 24 h the curve for carbon ions approached that for X rays, while the curve for iron ions remained high. These chromosome data reflect the existence of a slow NHEJ repair phase and severe biological damage induced by iron ions. The auto-phosphorylation of DNA-dependent protein kinase catalytic subunits (DNA-PKcs), an essential NHEJ step, was delayed significantly by high-LET carbon- and iron-ion radiation compared to X rays. This delay was further emphasized in NHEJ-defective 180BR cells. Our results indicate that high-LET radiation induces complex DNA damage that is not easily repaired or is not repaired by NHEJ even at low radiation doses such as 2 Gy.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0033-7587","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"442962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Maki"}],"nameIdentifiers":[{"nameIdentifier":"442963","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabe, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"442964","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Noguchi, Miho"}],"nameIdentifiers":[{"nameIdentifier":"442965","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakura, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"442966","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Sentaro"}],"nameIdentifiers":[{"nameIdentifier":"442967","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442968","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田 真希","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442969","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部 篤史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442970","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野口 実穂","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442971","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高倉 かほる","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442972","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 千太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442973","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the Non-homologous End-joining pathway","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the Non-homologous End-joining pathway"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-09-12"},"publish_date":"2006-09-12","publish_status":"0","recid":"44605","relation_version_is_last":true,"title":["Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the Non-homologous End-joining pathway"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:13:29.498320+00:00"}