{"created":"2023-05-15T14:34:29.323960+00:00","id":44561,"links":{},"metadata":{"_buckets":{"deposit":"cf7747da-a599-44fa-822e-c44205793096"},"_deposit":{"created_by":1,"id":"44561","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44561"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044561","sets":["1"]},"author_link":["442642","442633","442638","442639","442637","442629","442640","442641","442630","442631","442635","442634","442632","442636"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"181","bibliographicPageStart":"175","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the protective effects of melatonin against high-LET ionizing radiation, V79 Chinese hamster cells were irradiated with 100 keV/um carbon beam. Parallel experiments were performed with 200 kV X-rays. To avoid the impact from extra solvents, melatonin was dissolved directly in culture medium. Cells were cultured in melatonin medium for 1 hr before irradiation. Cell inactivation was measured with conventional colony forming assay, medium containing 6-thioguanine was used for the selection of mutants at hprt locus, and the cell cycle was monitored by flow cytometry. Both carbon beam and X-rays induced cell inactivation, hprt gene mutation and cell cycle G2 block dose-dependently. But carbon beam showed stronger effects as indicated by all three endpoints and the relative biological effectiveness (RBE) was 3.5 for cell killing (at 10% survival level) and 2.9 for mutation induction (at 5x10^-5 mutants/cell level). Melatonin showed protective effects against ionizing radiation in a dose-dependent manner. In terms of cell killing, melatonin only increased the survival level of those samples exposed to 8Gy or larger of X-rays or 6 Gy or larger of carbon beam. In the induction of hprt mutation and G2 block, melatonin reduced such effects induced by carbon beam but not by X-rays. The results suggest that melatonin reduces the direct interaction of particles with cells rather than an indirect interaction. Further studies are required to disclose the underlying mechanisms.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhou, Guangming"}],"nameIdentifiers":[{"nameIdentifier":"442629","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawata, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"442630","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"442631","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Mizuho"}],"nameIdentifiers":[{"nameIdentifier":"442632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Ryoichi"}],"nameIdentifiers":[{"nameIdentifier":"442633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"442634","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Hisao"}],"nameIdentifiers":[{"nameIdentifier":"442635","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"周 光明","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442636","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川田 哲也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442637","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442638","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 瑞穂","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442639","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山 亮一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442640","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安藤 興一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442641","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊東 久夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442642","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protective Effects of Melatonin Against Low- and High-LET Irradiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protective Effects of Melatonin Against Low- and High-LET Irradiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-08-10"},"publish_date":"2006-08-10","publish_status":"0","recid":"44561","relation_version_is_last":true,"title":["Protective Effects of Melatonin Against Low- and High-LET Irradiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:14:00.902809+00:00"}