{"created":"2023-05-15T14:34:27.672742+00:00","id":44524,"links":{},"metadata":{"_buckets":{"deposit":"bb8f0ddd-1711-4e7b-80e9-4efce4dfcf06"},"_deposit":{"created_by":1,"id":"44524","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44524"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044524","sets":["1"]},"author_link":["442416","442420","442418","442415","442422","442411","442414","442413","442412","442421","442423","442424","442417","442419","442410"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8890","bibliographicPageStart":"8882","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Cancer Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Deregulated V(D)J recombination-mediated chromosomal rearrangements are implicated in the etiology of B- and T-cell lymphomagenesis. We describe here three pathways for the formation of the 5' deletions of Notch1 gene in thymic lymphomas of wild-type or V(D)J recombination-defective scid mice. A pair of recombination signal sequence -like sequences composed of heptamer-like and nonamer-like motifs separated by 12- or 23-bp spacers (12- or 23-recombination signal sequence) were present in the vicinity of the deletion breakpoints in wild-type thymic lymphomas, accompanied by palindromic or nontemplated nucleotides at the junctions. In scid thymic lymphomas the deletions at the recombination signal sequence-like sequences occurred at a significantly lower frequency than in wild-type mice, whereas the deletions did not occur in Rag2-/- thymocytes. These results show that the 5' deletions are formed by Rag-mediated V(D)J recombination machinery at cryptic recombination signal sequences in the Notch1 locus. In contrast, one third of the deletions in radiation-induced scid thymic lymphomas had microhomology at both ends, indicating that in the absence of DNA-dependent protein kinase-dependent nonhomologous end-joining, the microhomology-mediated nonhomologous endjoining pathway functions as a main mechanism to produce deletions. Furthermore the deletions were induced via a coupled pathway between Rag-mediated cleavage at cryptic recombination signal sequence and micronomology-mediated end-joining in radiation-induced scid thymic lymphomas. As the deletions at cryptic recombination signal sequence occur spontaneously, microhomology-mediated pathways might participate mainly in in radiation-induced lymphomagenesis.  Recombination signal sequence-mediated deletions were present clonally in thymocyte population,  suggesting that thymocytes with a 5'deletion of the Notch1 gene have a growth advantage and are involved in lymphomagenesis.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0008-5472","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsuji, Hideo"}],"nameIdentifiers":[{"nameIdentifier":"442410","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii-Ohba, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"442411","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsube, Takanori"}],"nameIdentifiers":[{"nameIdentifier":"442412","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ukai, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"442413","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aizawa, Shirou"}],"nameIdentifiers":[{"nameIdentifier":"442414","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Doi, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"442415","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hioki, Kyoji"}],"nameIdentifiers":[{"nameIdentifier":"442416","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogiu, Toshiaki"}],"nameIdentifiers":[{"nameIdentifier":"442417","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442418","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 洋子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442419","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部 孝則","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442420","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜飼 英樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442421","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相澤 志郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442422","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"土居 雅広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442423","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荻生 俊昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"442424","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Involvement of Illegitimate V(D)J Recombination or Microhomology-Mediated Nonhomologous End-Joining in the Formation of Intragenic Deletions of the Notch1 Gene in Mouse Thymic Lymphomas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of Illegitimate V(D)J Recombination or Microhomology-Mediated Nonhomologous End-Joining in the Formation of Intragenic Deletions of the Notch1 Gene in Mouse Thymic Lymphomas"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-08-03"},"publish_date":"2006-08-03","publish_status":"0","recid":"44524","relation_version_is_last":true,"title":["Involvement of Illegitimate V(D)J Recombination or Microhomology-Mediated Nonhomologous End-Joining in the Formation of Intragenic Deletions of the Notch1 Gene in Mouse Thymic Lymphomas"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:14:27.621596+00:00"}